Bicyclic and tricyclic inhibitors of sumoylation enzymes and methods of their use

ABSTRACT

According to the embodiments described herein, a tricyclic SUMOylation inhibitor compound is provided. In some embodiments, a method for inhibiting a SUMOylation enzyme in a cell is provided. Such a method may include administering a SUMOylation inhibitor compound to the cell. In some aspects, the SUMOylation enzyme is SUMO E1 or SUMO E2. In some aspects, the method may be used to inhibit a cancer cell in vitro (e.g., grown in culture) or in vivo (e.g., as part of a tumor in a subject). In other embodiments, a method for treating a cancer, degenerative diseases and viral infection is provided. Such a method may include administering an effective amount of a pharmaceutical composition to a subject having the cancer. The pharmaceutical composition may include a SUMOylation inhibitor compound. In some embodiments, the method for treating a disease may further comprise administering one or more DNA-damaging therapy in combination with administration of the pharmaceutical composition.

RELATED APPLICATIONS

This application is a continuation application of PCT Application No.PCT/US2011/60063, filed Nov. 9, 2011, which claims the benefit of U.S.Provisional Application No. 61/411,855, filed Nov. 9, 2010, which arehereby incorporated in their entirety and for all purposes.

GOVERNMENT INTEREST

The invention was made with Government support under Grant Nos. R01GM086172, F32 CA134180, and R03DA026556-01 awarded by the NationalInstitutes of Health (NIH). The Government has certain rights in theinvention.

BACKGROUND

Post-translational modifications of cellular proteins by the smallubiquitin-like modifier (SUMO) family of proteins are importantepigenetic mechanisms for regulating various cellular functions. Atleast three members of the SUMO family (SUMO-1, -2, and -3) areubiquitin-like proteins that can conjugate to other cellular proteins bya biochemical mechanism similar to ubiquitination (Hay 2005; Sarge 2009;Yeh 2009).

SUMOylation requires multiple steps that are catalyzed by three types ofSUMOylation enzymes: activating enzyme E1 (made up of two subunits, SAE1and SAE2/Uba2), conjugating enzyme E2 (Ubc9), and one of several E3ligases. This pathway is illustrated for SUMO 1 in FIG. 1. Briefly, SUMOis activated by the E1 enzyme through ATP hydrolysis and forms athioester conjugate with E1. SUMO is then transferred to E2, forming athioester conjugate with E2. Finally, SUMO is transferred to targetproteins, a step usually stimulated by an E3 ligase. SUMO modificationadds a new docking site to target proteins, and thus enables newprotein-protein interactions through the SUMO-interacting motif (SIM) inreceptor proteins (Song 2004; Song 2005). The E1 and E2 enzymes do notdiscriminate among the different SUMO paralogues (Tatham 2003).

SUMOylation is reversible by a process known as deSUMOlyation. Theremoval of SUMO proteins from modified target proteins is accomplishedby deSUMOylation enzymes such as isopeptidase and SUMO/sentrin-specificprotease (SENP).

Aberrations in post-translational modification of cellular proteins bythe small ubiquitin-like modifier (SUMO) family of proteins areassociated with the pathogenesis of life-threatening diseases, such ascancer (Luo 2009; Kim 2006; Mo 2005), neurodegenerative disorders(Steffan 2004; Subramaniam 2009), and viral infection (Jaber 2009;Ulrich 2009; Kim 2010). Viral infection often involves hijacking thehost post-translational modifications, providing viruses with a fastmeans for becoming established in host cells before the immune systemcan respond.

SUMOylation and deSUMOylation enzymes regulate dynamic SUMOmodifications in controlling cellular functions. One of the predominantfunctions of SUMO-mediated modifications is in DNA damage response, suchas damage caused by chemo- and radiation therapy (CRT), which killscancer cells by inducing genotoxic stress (Galanty 2009; Morris 2009;Ouyang 2009; Prudden 2009; Li 2010). DNA double-strand breaks (DSBs) arethe most dangerous form of DNA damage, and lead to cell death if leftunrepaired (FIG. 2) (Darzynkiewicz 2009). Upon DSB formation, thehistone protein H2AX is phosphorylated, resulting in recruitment ofseveral DNA damage signaling proteins to the damage sites, includingp53-binding protein 1 (53BP1) and ATM (van Attikum 2009). SUMOylation isrequired for multiple steps in DNA repair pathways, includingrecruitment of signaling and repair proteins to damage sites andenablement of repair protein function. For example, recruitment of 53BP1to DNA damage sites is dependent on its SUMOylation (Galanty 2009).53BP1 mediates the signaling process to stop cell cycle progression andDNA replication and allows time for repair, in which p53 plays animportant role. p53 is also involved in apoptosis if DNA damage is notrepaired. SUMOylation also plays a role in regulating p53transactivating activity (Stehmeier 2009) and trafficking (Carter 2007).

SUMOylation also directly regulates repair of various types of DNAdamage. Recent studies have shown that SUMOylation is required for bothmajor DSB repair pathways: homologous recombination (HR), in which ahomologous sequence acts as a repair template, and non-homologous endjoining (NHEJ), in which DSB ends are ligated together (Jeggo 2009).Proteins involved in HR include the well-known breast cancer-relatedgenes BRCA1 and BRCA2, as well as other proteins with DNA binding andhelicase activities (Jeggo 2009). Proteins that carry out NHEJ includeKu70, Ku80, DNA-PKcs, XRCC4, XLF, and Artemis (Jeggo 2009). Manyproteins in the DSB repair pathways are substrates of SUMOylation (FIG.2) (Doksani 2009; Morris 2009; Bartek 2010; Li 2010). SUMOylation isalso important for response to single-stranded DNA damage (Pfander 2005)and nucleotide base excision repair (Steinacher 2005; Mohan 2007) bymodifying repair enzymes to regulate their activity and life spans.These findings suggest that inhibition of SUMO-dependent processes caninhibit repair of a wide range of DNA damage in cancer cells, therebysensitizing tumor cells to genotoxic stress induced by CRT.

SUMOylation is required for DNA repair, as evidenced by the observationthat cells defective in SUMOylation are sensitive to DNA damage reagents(al-Khodairy et al. 1995; Shayeghi et al. 1997). Recently, twoindependent studies have identified the yeast protein, Mms21, as theSUMO E3 ligase required for repair of both DNA alkylation damage anddouble-strand breaks (Andrews et al. 2005; Zhao & Blobel 2005).Elimination of Mms21's SUMO E3 activity leads to DNA damage sensitivity.However, the SUMOylation targets in the DNA damage response are not yetwell established, nor is SUMOylation's involvement in DNA repair orother cellular functions. Recent studies have shown that a SUMO-targetedubiquitin ligase (STUBL) is important in DNA damage response, and theligase specifically recognizes poly-SUMO-2/3 chains to ubiquitinatepoly-SUMO modified proteins for degradation (Burgess et al. 2007; Ii etal. 2007; Prudden et al. 2007; Nagai et al. 2008; Cook et al. 2009; Sunet al. 2007).

The enzymes catalyzing SUMO-modification (E1, E2, E3) are present inhigher levels in cancer tissues versus normal tissues and inmetastasized tumors versus normal cells, and play an important role incancer proliferation and metastasis. Recent studies suggest that E1presents an ideal target for the development of cancer therapeutics withspecific genetic backgrounds. For example, a genome-wide siRNA screenidentified the genes encoding the SUMO E1 subunits SAE1 and SAE2 amongthose genes with the strongest synthetic lethal interactions with KRas(Luo 2009).

DeSUMOylation enzymes are also thought to be important in cancer.Increased levels of a deSUMOylation isopeptidase (Senp1) have beenobserved in prostate cancer, and suppression of Senp1 level by siRNA hasbeen shown to suppress prostate cancer and angiogenesis. Hypoxia alsoinduces high levels of SUMO-1. SUMO-mediated protein-proteininteractions appear to be involved in most SUMO-dependent processes.

Given the role of SUMOylation in cancer and other disease states such asviral infection, there is a need in the art for novel SUMOylation enzymeinhibitors. Such inhibitors would be useful both as therapeutics and asresearch tools for studying the role of SUMOylation in cellularregulation.

SUMMARY

According to the embodiments described herein, a SUMOylation inhibitorcompound is provided. In one embodiment, the SUMOylation inhibitorcompound has a structure comprising:

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein:

-   -   A and B are independently selected from C, N or heteroatoms;    -   R₁ is H, alkyl, haloalkyl, alkyl-OR₁, aryl, heterocyclyl,        heteroaryl, benzyl, alkyl-aryl wherein R₁ is optionally        substituted with one to four R₄ groups;    -   R₂ is H or L-R₅, wherein L is —C(O)— or —S(O2)-, and R₅ is        alkyl, haloalkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl,        benzyl, alkyl-aryl where R₅ is optionally substituted with one        to four R₄ groups;    -   R₃ is CN or —C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl,        haloalkyl, aryl, heteroaryl where R₅ is optionally substituted        with one to four R₄ groups; and    -   R₄ is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂, —S(O₂)N(R⁷)₂,        —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,        —C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,        —N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl,        alkyl-OR⁷, or alkyl-N(R⁷)₂, where each R⁷ is independently of H,        alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl or heteroaryl.

In another embodiment, the SUMOylation inhibitor compound has astructure comprising:

and pharmaceutically acceptable derivatives, solvates, salts andstereoisomers thereof;

-   -   wherein R₁ and R₂ are independently selected from the group        consisting of H, —S(O)₂—R₈, —CN and —C(O)—X—R₉;    -   wherein R₈ is an alkyl or aryl;    -   wherein R₉ is an alkyl, aryl, or heteroaryl;    -   wherein X is selected from the group consisting of C, O, N, S or        P;    -   wherein R₃ is selected from the group consisting of an alkyl, an        alkenyl, and an alkylaryl;    -   wherein R₄, R₅, R₆ and R₇ are selected from the group consisting        of H, a halogen, and an alkyl;    -   wherein X₁ is selected from the group consisting of C, N, O, S,        and P; and    -   wherein X₂, X₃ and X₄ are selected from the group consisting of        N and O.

In another embodiment, the SUMOylation inhibitor compound has astructure comprising:

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein:

-   -   A and B are independently selected from the group consisting of        C, N or heteroatoms;    -   R₁ is H, alkyl, haloalkyl, alkyl-OR₁, aryl, heterocyclyl,        heteroaryl, benzyl, alkyl-aryl where R₁ is optionally        substituted with one to four R₄ groups;    -   R₂ is H or L-R₅, wherein L is —C(O)— or —S(O2)-, and R₅ is        alkyl, haloalkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl,        benzyl, alkyl-aryl where R₅ is optionally substituted with one        to four R₄ groups;    -   R₃: is CN or —C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl,        haloalkyl, aryl, heteroaryl where R₅ is optionally substituted        with one to four R₄ groups; and    -   R₄: is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂, —S(O₂)N(R⁷)₂,        —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,        —C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,        —N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl,        alkyl-OR⁷, or alkyl-N(R)₂, where each R⁷ is independently of H,        alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl or heteroaryl.

In another embodiment, the SUMOylation inhibitor compound has astructure comprising:

wherein R₁ is selected from CN, SO₂Tol or CONHBu; R₂ is selected fromCOAr or SO₂Ph; and R₃ is selected from Bu, Bn or allyl. In certainembodiments, the SUMOylation inhibitor compound may be selected from thegroup having the structure of:

In some embodiments, a method for inhibiting a SUMOylation enzyme in acell is provided. Such a method may include administering a SUMOylationinhibitor compound, such as those described above, to the cell. In someaspects, the SUMOylation enzyme is SUMO E1 or SUMO E2.

In other embodiments, a method for treating a disease is provided. Sucha method may include administering an effective amount of apharmaceutical composition to a subject having the cancer. Thepharmaceutical composition may include a SUMOylation inhibitor compound,such as those described above. In some aspects of this embodiment, thecancer may be any cancer associated with an overexpression orunderexpression of a SUMO or SUMOylation enzyme or is associated withSUMOylation of a specific protein. Examples of diseases that may betreated in accordance to the embodiments described herein may include,but are not limited to, cancer and other neoplastic conditions (e.g.,colorectal cancer, pancreatic cancer, bone cancer or breast cancer),viral infections (e.g., HIV), hereditary diseases and degenerativediseases.

In some embodiments, the method for treating a cancer may furthercomprise administering one or more DNA-damaging therapy in combinationwith administration of the pharmaceutical composition. Examples of DNAdamaging therapies that may be administered in accordance with theembodiments of the disclosure include, but are not limited to, anionizing radiation source or a chemotherapeutic agent selected from analkylating agent, platinum analogue or other alkylating-like ornonclassical alkylating agent; an intercalating agent; a topoisomeraseinhibitor; or a cytotoxic antibiotic.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 illustrates the three aspects of the SUMO-mediated processes asthey relate to human health. The enzymatic pathway of SUMOylation and adiagram of SUMO-dependent protein complex formation.

FIG. 2 illustrates the role of SUMOylation in response to a DNAdouble-strand break in response to chemo- and radiation therapy (CRT). Aschematic of current knowledge of the DNA damage signaling and repairpathways for DSBs is shown. Red ovals mark proteins known to be modifiedby SUMO-1, SUMO-2 or SUMO-3. SUMOylation not only controls eventsupstream of the repair pathways, but is important for both HR and NHEJrepair pathways. Although not depicted here, SUMOylation is alsoimportant for single-stranded DNA repair, regulating nuclease stability,inhibition of the formation of cruciform DNA upon damage, and nucleotidebase excision repair.

FIG. 3 illustrates the characterization of the tricyclic probe used togenerate SUMO enzyme inhibitors. (A) Quinoxaline analogs synthesized andtested. (B) Compound 1 is one of the most potent analogues from thisseries. Compound 2 is a similar compound, but lacks the Ph-group and isnot inhibitory to the SUMO E1. Therefore, it is used as a control.

FIG. 4 shows analogues of the first class of SUMO E1 inhibitor toexplore the three R-groups. The analogues and their IC50 values ininhibiting the SUMO E1 in biochemical assays are indicated below eachanalogue.

FIG. 5 shows that compound 1 has a highly selective inhibitory effect onSUMOylation and on a SUMO E1 enzyme, while showing that theubiquitination pathway is not inhibited. The upper panel shows formationof the Ubc9˜SUMO thioester, which is catalyzed by the SUMO E1, at theindicated concentrations of inhibitor. The IC₅₀ is approximately 0.5 μM.In contrast, the inhibitor has an IC₅₀ value >100-fold higher forinhibiting the ubiquitin E1 during formation of Ubc5˜ubiquitin thioester(lower panel). All protein levels were calibrated using amino acidanalysis and that the visual difference of Ubc9 versus Ubc5 level is dueto Coomassie staining differences.

FIG. 6 shows STD NMR experiments of ATP and compound 1 in the presenceof the SUMO E1. In (A), ATP spectra with assignments indicated, and thepercentage of STD effects indicated for each resonance. Red spectrumindicates 2 s saturation at −30 ppm (control), blue spectrum (underneathred) indicates 2 s saturation of protein signals at 0 ppm, and greenspectrum (bottom) indicates the difference between the red and bluespectra. H2 experiences the largest STD effect followed by H1′,consistent with the X-ray structure (Lois & Lima 2005). In (B), STD of60 μM ATP upon titration of compound 1 at the concentrations indicatedto the right. The corresponding STD spectra are color-coded as indicatedto the right. A region of the spectrum covering H2 of ATP and H12 andH13 from the compound are shown, with the H2 signal of ATP expanded inthe inset. The compound has strong STD effects, and titration of 1reduced ATP STD effect in a concentration dependent manner, indicatingstrong competition with ATP binding. The titration data was used toestimate K_(d) of the compound.

FIG. 7 illustrates that compound 1 inhibits global SUMOylation in adose-dependent manner. Western blots (probed with anti-SUMO2/3antibodies) of cell lysates from HT29, HCT116 and U373 cells treatedwith compound 1. SUMO2/3 modifies many proteins as poly-SUMO chains, andis detected as high molecular weight smears. 16c indicates the controlcompound 2 used at concentrations of 16 μM.

FIG. 8 shows sensitization of breast cancer (MCF7), non-small cell lungcancer (H1299), colorectal cancer (DLD1) and bone cancer (SJSA) celllines treated with tricyclic SUMOylation inhibitor MLS-0207587 inresponse to irradiation. The cells were treated with different doses ofthe drug for 4 hours, followed by irradiation with 4 Gy and incubationfor an additional 68 hours. Compared with cells treated only with thedrug, the cells treated with both the drug and radiation had asignificantly lower survival rate, validating the sensitization effectof the SUMO inhibitor. Cell survival was assessed by MTS assays

FIG. 9 illustrates the toxicity of the SUMO inhibitor on normal humanliver cells. Immortalized human hepatocytes, Fa2N-4 cells (XenoTech)were seeded at ˜50-60K cells/well, and incubated with a range ofconcentrations (0.01-50 μM) 1, in duplicate, for 24 hrs at 37° C., 5%CO2. Cell viability was determined by cellular ATP levels using theLuminescence ATP Detection Assay System (Tecan).

FIG. 10 illustrates a representative assay to determine the efficacy ofSUMO inhibitors generated from the tricyclic probe shown in FIG. 5.

FIG. 11 illustrates a dose-response in thioester formation assays todetermine the effective dose of a candidate tricyclic SUMO inhibitor(MLS-0033005).

FIG. 12 illustrates a dose-response in thioester formation assays todetermine the effective dose of a candidate tricyclic SUMO inhibitor(MLS-0435780).

FIG. 13 illustrates a dose-response in thioester formation assays todetermine the effective dose of a candidate tricyclic SUMO inhibitor(MLS-0435784).

FIG. 14 shows that tricyclic SUMO inhibitor compound 1 (quinoxaline)inhibits HIV infection in a dose-dependent manner.

FIG. 15 illustrates that MLS-0207587 (shown as SU2) does not disruptstable SUMOylation in U2OS cells and DLD1 cells (Green: RanGAP1;Red:RanBP2). Cells were treated with the compound at indicatedconcentration for 18 hours, and then localization of RanGAP1 and RanBP2was detected by immunohistochemistry. Nuclei are stained with DAPI.

FIG. 16 illustrates that MLS-0207587 inhibits 53BP1 colocalization withγH2AX at DSB sites in IR-treated DLD1 cells. (A) shows colocalization of53BP1 and γH2AX (lower left panel) in untreated irradiated DLD1 cells,while (B) shows a lack of colocalization as illustrated by a lack offoci when the DLD1 cells are treated with 20 μM MLX-0207587 for 18 hoursfollowed by irradiation at 1 Gy followed by 1 hour recovery period.(Green: γH2AX; Red: 53BP1).

FIG. 17 illustrates that MLS-0207587 inhibits 53BP1 colocalization withγH2AX at DSB sites in IR-treated U2OS cells. (A) shows colocalization of53BP1 and γH2AX (lower left panel) in untreated irradiated U2OS cells,while (B) shows a lack of colocalization as illustrated by a lack offoci when the U2OS cells are treated with 20 μM MLS-0207587 for 18 hoursfollowed by irradiation using 1 Gy, followed by 1 hour recovery. (Green:γH2AX; Red: 53BP1).

FIG. 18 illustrates that MLS-0207587 inhibits BRCA1 colocalization withγH2AX in IR-treated DLD1 cells. (A) shows colocalization of BRCA1 andγH2AX (lower left panel) in untreated irradiated DLD1 cells, while (B)shows a lack of colocalization as illustrated by a lack of foci when theDLD1 cells are treated with 20 μM MLS-0207587 for 18 hours followed byirradiation at 1 Gy followed by 1 hour recovery. (Green: BRCA1; Red:γH2AX).

FIG. 19 illustrates that MLS-0207587 inhibits BRCA1 colocalization withγH2AX in IR-treated U2OS cells. (A) shows colocalization of BRCA1 andγH2AX (lower left panel) in untreated irradiated U2OS cells, while (B)shows a lack of colocalization as illustrated by a lack of foci when theU2OS cells are treated with 20 μM MLS-0207587 for 18 hours followed byirradiation (1 Gy; 1 hour). (Green: BRCA1; Red: γH2AX).

FIGS. 20A-C are a series of tables illustrating SUMO inhibitors thatwere developed in accordance with some embodiments of the tricyclicscaffolds described herein.

DETAILED DESCRIPTION

Provided herein are small molecule inhibitors of SUMOylation enzymes orpharmaceutically acceptable derivatives thereof, as well as variousmethods of identifying, making and using these inhibitors. Uses for theSUMOylation enzyme inhibitors described herein include, but are notlimited to, methods for modulating radiation sensitivity of cancercells, killing cancer cells and treating diseases and conditions such ascancer and other neoplastic conditions, viral infections, hereditarydiseases and degenerative diseases.

The term “SUMOylation inhibitor” or “SUMO inhibitor” as used hereinrefers to any small molecule inhibitor that binds one or more subunit ofa SUMOylation enzyme, thereby inhibiting the addition of a SUMO proteinto a target protein. Such small molecule inhibitors may also inhibit oneor more SUMOylation enzymes. The SUMOylation inhibitors, as furtherdescribed in the studies described herein, have a high level ofspecificity to SUMO enzymes, thereby affecting SUMOylation, but do notbind or have very low level or negligible binding to proteins found inthe ubiquitination pathway. The term “SUMOylation enzyme” or “SUMOenzyme” as used herein refers to SUMO activation enzyme E1, SUMOconjugating enzyme E2 or any one or more of approximately ten SUMO E3ligases.

In some embodiments, the SUMO inhibitors described herein are SUMO E1inhibitors. The term “SUMO E1” as used herein refers to SUMO activatingenzyme E1, which is made up of subunits SAE1 and SAE2/Uba2. In certainembodiments, the small molecule SUMO E1 inhibitors disclosed hereininhibit the SAE2 subunit of E1, by interfering with the active site orby acting allosterically. The SUMO E1 inhibitors may inhibit SUMO E2.The term “SUMO E2” as used herein refers to SUMO conjugating enzyme E2,which is made up of a single subunit, Ubc9. In certain embodiments, thesmall molecule SUMO inhibitors described herein inhibit Ubc9 only, orthe inhibitors may inhibit Ubc9 and one or more E1 subunits. In theseembodiments, the inhibitors may inhibit Ubc9 to a greater degree thanthe one or more E1 subunits, or they may inhibit two or more of thesubunits equally.

Increased expression of SUMO enzymes may contribute to cancer, tumors,or other neoplastic conditions, viral infection, degenerative diseases,genetic or hereditary diseases, or other pathological conditions ordiseases. Many cancers have been shown to be associated with increasedlevels of SUMO enzymes. For example, as discussed in detail in theExamples below, SUMO E1 was found to be the most elevated SUMO enzyme incolorectal cancer tissue, and these high levels of SUMO E1 wereassociated with radioresistance in colorectal cancer as well as severalother types of cancer (Wiatrek et al. Differential expression of smallubiquitin-like modifier family of proteins in patients with colorectaladenocarcinoma; ASCO Abstract, 2011, which is hereby incorporated byreference, as if fully set forth herein; seehttp://www.asco.org/ascov2/Meetings/Abstracts?&vmview=abst_detail_view&confide=103&abstractID=71189). Overexpression of SUMO enzymes have alsobeen observed in other cancer types (Zhu et al. 2010; Kim et al. 2006;Comerford et al 2003; Cheng et al. 2007; Bergink & Jentsch 2009; Galantyet al. 2009; Morris et al. 2009; Ouyang et al. 2009; Subramaniam et al.2009; Steffan et al. 2004; Jaber et al. 2009)

Colorectal cancer is the second leading cause of cancer death in theUnited States (Burt 2009), and CRT is frequently used against colorectalcancer as a preoperative treatment to facilitate surgical intervention(Watanabe 2008) and improve long-term survival (Garcia-Aguilar 2003).However, only a small percentage (<15%) of patients have a completeresponse to CRT. Therefore, novel SUMO E1 inhibitors that enhance CRTeffects and/or impair tumor viability are needed to improve treatmentoutcomes, preserve quality of life, and reduce healthcare costs. SuchSUMO E1 inhibitors may be similarly useful in other cancers, diseasesand conditions associated with overexpression of SUMO E1 (Zhu et al.2010; Kim et al. 2006; Comerford et al 2003; Cheng et al. 2007; Bergink& Jentsch 2009; Galanty et al. 2009; Morris et al. 2009; Ouyang et al.2009; Subramaniam et al. 2009; Steffan et al. 2004; Jaber et al. 2009).

Therefore, as disclosed herein, a set of small molecule SUMO E1inhibitors (or “SUMO inhibitor compounds”) have been identified andcharacterized. These inhibitors, which bind to SUMO E1 with highaffinity and specificity, represent the first highly specific smallmolecule inhibitors of SUMO E1. In some embodiments, the SUMO inhibitorsdo not bind or have negligible binding affinity and specificy forproteins in the ubiquitination pathway. Based on this disclosure,provided herein in certain embodiments are small molecule inhibitors ofSUMO E1.

Bicyclic Scaffolds

In certain embodiments, the small molecule SUMO inhibitors ofpharmaceutically acceptable derivatives thereof provided herein comprisea bicyclic scaffold. The bicyclic scaffold may comprise a structure ofStructure A as set forth below:

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein:

-   -   A and B are independently selected from C, N or heteroatoms;    -   R₁ is H, alkyl, haloalkyl, alkyl-OR₁, aryl, heterocyclyl,        heteroaryl, benzyl, alkyl-aryl wherein R₁ is optionally        substituted with one to four R₄ groups;    -   R₂ is H or L-R₅, wherein L is —C(O)— or —S(O2)-, and R₅ is        alkyl, haloalkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl,        benzyl, alkyl-aryl where R₅ is optionally substituted with one        to four R₄ groups;    -   R₃ is CN or —C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl,        haloalkyl, aryl, heteroaryl where R₅ is optionally substituted        with one to four R₄ groups; and    -   R₄ is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂, —S(O₂)N(R⁷)₂,        —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,        —C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,        —N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl,        alkyl-OR⁷, or alkyl-N(R⁷)₂, where each R⁷ is independently of H,        alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl or heteroaryl.

Tricyclic Scaffolds

In certain embodiments, the small molecule SUMO inhibitors orpharmaceutically acceptable derivatives thereof provided herein comprisea tricyclic scaffold. The tricyclic scaffold may comprise a structure ofStructure B as set forth below:

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein:

-   -   R₁ and R₂ are independently selected from the group consisting        of H, —S(O)₂—R₈, —CN and —C(O)—X—R₉;    -   R₈ is alkyl or aryl;    -   R₉ is alkyl, aryl, or heteroaryl;    -   X is selected from the group consisting of nothing, O, N, S or        P;    -   R₃ is selected from the group consisting of alkyl, alkenyl, and        alkylaryl;    -   R₄, R₅, R₆ and R₇ are independently selected from the group        consisting of H, halogen, and alkyl;    -   X₁ is selected from the group consisting of C, N, O, S, and P;        and    -   X₂, X₃ and X₄ are independently selected from the group        consisting of N, C, O.

In one embodiment, the compound comprising a structure of Structure B,and the pharmaceutically acceptable derivative, solvates, salts andstereoisomers thereof, including mixtures thereof in all ratios,wherein:

-   -   R₁ and R₂ are independently selected from the group consisting        of —S(O)₂—R₈, —CN and —C(O)—X—R₉;    -   R₈ is unsubstituted phenyl or phenyl substituted with alkyl,        alkenyl, alkenyl, halo, or alkoxyl;    -   R₉ is methyl, furanyl, substituted or unsubstituted phenyl;    -   X is N for R₁, and nothing for R₂;    -   R₃ is butyl, 1- or 2-propenyl, benzyl, or phenyl;    -   R₄ and R₇ are H;    -   R₅ and R₆ are methyl;    -   X₁ is C or N; and    -   X₂, X₃ and X₄ are N.

In a more preferred embodiment, the compound comprising a structure ofStructure B, and the pharmaceutically acceptable derivative, solvates,salts and stereoisomers thereof, including mixtures thereof in allratios, wherein:

-   -   R₁ and R₂ are independently selected from the group consisting        of —S(O)₂—R₈, —CN and —C(O)—X—R₈;    -   R₈ is phenyl or phenyl substituted with methyl, fluoro, chloride        or iodide;    -   R₉ is methyl, furanyl, phenyl or phenyl substituted with methyl,        fluoro, or chloride;    -   X is O;    -   R₃ is butyl or phenyl;    -   R₄ and R₅ are H;    -   R₆ and R₇ are methyl;    -   X₁ is N; and    -   X₂, X₃ and X₄ are N.

In another embodiment, the tricyclic scaffold may comprise a structureof Structure C as set forth below:

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein:

-   -   A and B are independently selected from the group consisting of        C, N or heteroatoms;    -   R₁ is H, alkyl, haloalkyl, alkyl-OR₁, aryl, heterocyclyl,        heteroaryl, benzyl, alkyl-aryl where R₁ is optionally        substituted with one to four R₄ groups;    -   R₂ is H or L-R₅, wherein L is —C(O)— or —S(O2)-, and R₅ is        alkyl, haloalkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl,        benzyl, alkyl-aryl where R₅ is optionally substituted with one        to four R₄ groups;    -   R₃: is CN or —C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl,        haloalkyl, aryl, heteroaryl where R₅ is optionally substituted        with one to four R₄ groups; and    -   R₄: is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂, —S(O₂)N(R⁷)₂,        —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,        —C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,        —N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl,        alkyl-OR⁷, or alkyl-N(R)₂, where each R⁷ is independently of H,        alkyl, haloalkyl, cycloalkyl, heterocyclyl, aryl or heteroaryl.

In certain of these embodiments the inhibitors comprise a quinoxalinescaffold having a structure as set forth below, and the pharmaceuticallyacceptable derivative, solvates, salts and stereoisomers thereof,including mixtures thereof in all ratios:

According to some embodiments, the functional groups of the TricyclicQuinoxaline scaffold, R₁, R₂ and R₃, are selected according theirability to increase the inhibitory activity of the scaffold. In someembodiments, R₁ is selected from CN, CO2R, CONHR, Tosyl (Ts), SO₂Tol orCONHBu. In other embodiments, R₂ is selected from H, COAr or SO₂Ph. Inother embodiments, R₃ is selected from n-Bu, n-Pent, CH₂CH₂OMe, Cy, orBn. Moreover, the R₁, R₂ and R₃ groups may be modified based onidentification of a functional group that exhibits the same, similar orenhanced binding affinity toward a SUMO enzyme binding site as comparedto the SUMO inhibitors described herein. In certain of these embodimentsthe SUMO inhibitors comprise a structure selected from one or more ofthe following structures and the pharmaceutically acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, set forth in Table 1 below:

TABLE 1 Tricyclic SUMO E1 inhibitors

Tricyclic Compound 1 MLS-0207587 (Quinoxaline) IC₅₀ = 0.5 μM

Tricyclic Compound 2 MLS-0435780 IC₅₀ = 20 μM

Tricyclic Compound 3 MLS-0033005 IC₅₀ = 3.6 μM

Tricyclic Compound 4 IC₅₀ = 8.3 μM

Tricyclic Compound 5 IC₅₀ = 25 μM

Tricyclic Compound 6 IC₅₀ = 64 μM

Tricyclic Compound 7 IC₅₀ = 98 μM

Tricyclic Compound 8 IC₅₀ = 4.5 μM

Tricyclic Compound 9 IC₅₀ = 1.1 μM

Tricyclic Compound 10 MLS-0435784 IC₅₀ = 5.7 μM

Tricyclic Compound 11 IC₅₀ > 100 μM

Tricyclic Compound 12 IC₅₀ = 0.5 μM

Tricyclic Compound 13 MLS-0048297 IC₅₀(-BSA) = 0.76 ± 0.07 μM

As used herein, the term “alkyl” refers to a straight or branched chainhydrocarbon having from one to twelve carbon atoms, optionallysubstituted with substituents selected from the group consisting ofC1-C6 alkyl, C1-C6 alkoxy, C1-C6 alkylsulfanyl, C1-C6 alkylsulfenyl,C1-C6 alkylsulfonyl, oxo, hydroxy, mercapto, amino optionallysubstituted by alkyl, carboxy, carbamoyl optionally substituted byalkyl, aminosulfonyl optionally substituted by alkyl, nitro, cyano,halogen, C1-C6 perfluoroalkyl or C1-C6 perfluoroalkoxy, multiple degreesof substitution being allowed. Examples of “alkyl” as used hereininclude, but are not limited to, methyl, ethyl, n-propyl, isopropyl,n-butyl, isobutyl, t-butyl, n-pentyl, isopentyl, and the like.

As used herein, when the term “C1-C6” is used to describe a group, itrefers to the group containing at least 1, and at most 6, carbon atoms.For example, the term “C1-C6 alkyl” include, but are not limited to,methyl, ethyl, n-propyl, isopropyl, isobutyl, n-butyl, t-butyl, n-pentyland isopentyl.

As used herein, the term “alkenyl” refers to a straight or branchedchain hydrocarbon having from two to twelve carbon atoms and at leastone carbon-carbon double bond, optionally substituted with substituentsselected from the group consisting of C1-C6 alkyl, C1-C6 alkoxy, C1-C6alkylsulfanyl, C1-C6 alkylsulfenyl, C1-C6 alkylsulfonyl, oxo, hydroxy,mercapto, amino optionally substituted by alkyl, carboxy, carbamoyloptionally substituted by alkyl, aminosulfonyl optionally substituted byalkyl, nitro, cyano, halogen, C1-C6 perfluoroalkyl or C1-C6perfluoroalkoxy, multiple degrees of substitution being allowed.Examples of “alkenyl” as used herein include, but are not limited to,ethenyl, n-propenyl, isopropenyl, n-butenyl, isobutenyl, t-butenyl,n-pentenyl, isopentenyl, and the like.

As used herein, the term “alkynyl” refers to a straight or branchedchain hydrocarbon having from two to twelve carbon atoms and at leastone carbon-carbon triple bond, optionally substituted with substituentsselected from the group consisting of C1-C6 alkyl, C1-C6 alkoxy, C1-C6alkylsulfanyl, C1-C6 alkylsulfenyl, C1-C6 alkylsulfonyl, oxo, hydroxy,mercapto, amino optionally substituted by alkyl, carboxy, carbamoyloptionally substituted by alkyl, aminosulfonyl optionally substituted byalkyl, nitro, cyano, halogen, C1-C6 perfluoroalkyl or C1-C6perfluoroalkoxy, multiple degrees of substitution being allowed.Examples of “alkyl” as used herein include, but are not limited to,ethynyl, n-propynyl, n-butynyl, isobutynyl, t-butynyl, n-pentynyl,isopentynyl, and the like.

As used herein, the term “halogen” or “hal” refers to fluorine (F),chlorine (Cl), bromine (Br) or iodine (I).

As used herein, the term “aryl” refers to an optionally substitutedbenzene ring or to an optionally substituted benzene ring system fusedto one or more optionally substituted benzene rings to form, forexample, anthracene, phenanthrene, or napthalene ring systems. Exemplaryoptional substituents include C1-C6 alkyl, C1-C6 alkoxy, C1-C6alkylsulfanyl, C1-C6 alkylsulfenyl, C1-C6 alkylsulfonyl, oxo, hydroxy,mercapto, amino optionally substituted by alkyl, carboxy, tetrazolyl,carbamoyl optionally substituted by alkyl, aminosulfonyl optionallysubstituted by alkyl, acyl, aroyl, heteroaroyl, acyloxy, aroyloxy,heteroaroyloxy, alkoxycarbonyl, nitro, cyano, halogen, C1-C6perfluoroalkyl or C1-C6 perfluoroalkoxy, heteroaryl, or aryl, multipledegrees of substitution being allowed. Examples of “aryl” groupsinclude, but are not limited to phenyl, 2-naphthyl, 1-naphthyl,biphenyl, as well as substituted derivatives thereof.

As used herein, the term “heteroaryl” refers to a monocyclic five toseven-membered aromatic ring, or to a fused bicyclic aromatic ringsystem comprising two of such monocyclic five to seven-membered aromaticrings. These heteroaryl rings contain one or more nitrogen, sulfurand/or oxygen heteroatoms, where N-oxides and sulfur oxides and dioxidesare permissible heteroatom substitutions and may be optionallysubstituted with up to three members selected from a group consisting ofC1-C6 alkyl, C1-C6 haloalkyl, C1-C6 haloalkoxy, C1-C6 alkoxy, C1-C6alkylsulfanyl, C1-C6 haloalkylsulfanyl, C1-C6 alkylsulfenyl, C1-C6alkylsulfonyl, oxo, hydroxy, mercapto, amino optionally substituted byalkyl, carboxy, tetrazolyl, carbamoyl optionally substituted by alkyl,aminosulfonyl optionally substituted by alkyl, acyl, aroyl, heteroaroyl,acyloxy, aroyloxy, heteroaroyloxy, alkoxycarbonyl, nitro, cyano,halogen, C1-C6 perfluoroalkyl or C1-C6 perfluoroalkoxy, heteroaryl oraryl, multiple degrees of substitution being allowed. Examples of“heteroaryl” groups used herein include furanyl, thiophenyl, pyrrolyl,imidazolyl, pyrazolyl, triazolyl, tetrazolyl, thiazolyl, oxazolyl,isoxazolyl, oxadiazolyl, oxo-pyridyl, thiadiazolyl, isothiazolyl,pyridyl, pyridazyl, pyrazinyl, pyrimidyl, quinolinyl, isoquinolinyl,benzofuranyl, benzothiophenyl, indolyl, indazolyl, and substitutedversions thereof.

As used herein, the term “haloalkyl” refers to an alkyl group as definedabove containing carbon atoms substituted with at least one halogen,halogen being as defined herein. Examples of branched or straightchained “C1-C6 haloalkyl” groups useful in the present inventioninclude, but are not limited to, methyl, ethyl, propyl, isopropyl,isobutyl and n-butyl substituted independently with one or morehalogens, e.g., fluoro, chloro, bromo and iodo.

As used herein, the term “alkoxy” refers to the group RaO—, where Ra isalkyl as defined above. Exemplary C1-C6 alkoxy groups useful in theinvention include, but are not limited to methoxy, ethoxy, n-propoxy,isopropoxy, n-butoxy and t-butoxy.

As used herein, the term “haloalkoxy” refers to the group RbO—, whereinRb is haloalkyl as defined above.

As used herein, the term “alkylsulfanyl” refers to the group R_(A)S—,where R_(A) is alkyl as defined above.

As used herein, the term “haloalkylsulfanyl” refers to the groupR_(D)S—, where R_(D) is haloalkyl as defined above.

As used herein, the term “alkylsulfenyl” refers to the group R_(A)S(O)—,where R_(A) is alkyl as defined above.

As used herein, the term “alkylsulfonyl” refers to the group R_(A)SO₂—,where R_(A) is alkyl as defined above.

As used herein, the term “oxo” refers to the group ═O.

As used herein, the term “mercapto” refers to the group SH.

As used herein, the term “carboxy” refers to the group COOH.

As used herein, the term “cyano” refers to the group CN.

As used herein, the term “cyanoalkyl” refers to the group R_(B)CN,wherein R_(B) is alkylen as defined above. Exemplary “cyanoalkyl” groupsuseful in the invention include, but are not limited to, cyanomethyl,cyanoethyl and cyanoisopropyl.

As used herein, the term “aminosulfonyl” refers to the group SO₂NH₂.

As used herein, the term “carbamoyl” refers to the group C(O)NH₂.

As used herein, the term “sulfanyl” shall refer to the group S—.

As used herein, the term “sulfenyl” shall refer to the group S(O)—.

As used herein, the term “sulfonyl” shall refer to the group S(O)₂— orSO₂.

As used herein, the term “acyl” refers to the group R_(F)C(O)—, whereR_(F) is alkyl, cycloalkyl or heterocyclyl as defined herein.

As used herein, the term “C3-C7 cycloalkyl” refers to a non-aromaticcyclic hydrocarbon ring having from three to seven carbon atoms andwhich optionally includes a C1-C6 alkyl linker through which it may beattached. The C1-C6 alkyl group is as defined above. Exemplary “C3-C7cycloalkyl” groups include, but are not limited to, cyclopropyl,cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl.

As used herein, the term “heterocyclic” or the term “heterocyclyl”refers to a three to twelve-membered heterocyclic ring having one ormore degrees of unsaturation containing one or more heteroatomicsubstitutions selected from S, SO, SO₂, O or N, optionally substitutedwith substituents selected from the group consisting of C1-C6 alkyl,C1-C6 haloalkyl, C1-C6 haloalkoxy, C1-C6 alkoxy, C1-C6 alkylsulfanyl,C1-C6 haloalkylsulfanyl, C1-C6 alkylsulfenyl, C1-C6 alkylsulfonyl, oxo,hydroxy, mercapto, amino optionally substituted by alkyl, carboxy,carbamoyl optionally substituted by alkyl, aminosulfonyl optionallysubstituted by alkyl, nitro, cyano, halogen, C1-C6 perfluoroalkyl orC1-C6 perfluoroalkoxy, multiple degrees of substitution being allowed.Such a ring may be optionally fused to one or more other “heterocyclic”ring(s) or cycloalkyl ring(s). Examples of “heterocyclic” moietiesinclude, but are not limited to, tetrahydrofuran, pyran, 1,4-dioxane,1,3-dioxane, pyrrolidine, piperidine, morpholine, tetrahydrothiopyran,tetrahydrothiophene, and the like.

As used herein, the term “aroyl” refers to the group R_(C)C(O)—, whereR_(C) is aryl as defined herein.

As used herein, the term “heteroaroyl” refers to the group R_(E)C(O)—,where R_(E) is heteroaryl as defined herein.

As used herein, the term “alkoxycarbonyl” refers to the groupR_(A)OC(O)—, where RA is alkyl as defined herein.

As used herein, the term “acyloxy” refers to the group R_(F)C(O)O, whereR_(F) is alkyl, cycloalkyl, or heterocyclyl as defined herein.

As used herein, the term “aroyloxy” refers to the group R_(C)C(O)O—,where R_(C) is aryl as defined herein.

As used herein, the term “heteroaroyloxy” refers to the groupR_(E)C(O)O—, where R_(E) is heteroaryl as defined herein.

As used herein, the term “carbonyl” or “carbonyl moiety” refers to thegroup C═O.

As used herein, the term “thiocarbonyl” or “thiocarbonyl moiety” refersto the group C═S.

As used herein, the term “amino,” “amino group” or “amino moiety” refersto the group NR_(G)R_(G)′, wherein R_(G) and R_(G)′, are preferablyselected, independently from one another, from the group consisting ofhydrogen, alkyl, haloalkyl, haloalkoxy, alkenyl, cycloalkyl,alkylenecycloalkyl, cyanoalkyl, aryl, aralkyl, heteroaryl, acyl andaroyl. If both R_(G) and R_(G)′ are hydrogen, NR_(G)R_(G)′ is alsoreferred to as “unsubstituted amino moiety” or “unsubstituted aminogroup.” If R_(G) and/or R_(G)′ are other than hydrogen, NR_(G)R_(G)′ isalso referred to as “substituted amino moiety” or “substituted aminogroup.”

As used herein, the term “imino” or “imino moiety” refers to the groupC═NR_(G), wherein R_(G) is preferably selected from the group consistingof hydrogen, alkyl, haloalkyl, haloalkoxy, alkenyl, cycloalkyl,alkylenecycloalkyl, cyanoalkyl, aryl, aralkyl, heteroaryl, acyl andaroyl. If R_(G) is hydrogen, C═NR_(G) is also referred to as“unsubstituted imino moiety.” If R_(G) is a residue other than hydrogen,C═NRG is also referred to as “substituted imino moiety.”

As used herein, the term “perfluoroalkyl” refers to an alkyl group withall hydrogens replaced by fluorine, e.g. without limitation, —CF₃.

As used herein, the term “perfluoroalkoxy” refers to an alkoxyl groupwith all hydrogens replaced by fluorine, e.g. without limitation, —OCF₃.

As used herein, “

” refers to a single bond or double bond.

As used herein, the terms “group,” “residue” and “radical” or “groups,”“residues” and “radicals” are usually used as synonyms, respectively, asit is common practice in the art.

Method for Inhibiting a SUMOylation Enzyme

In some embodiments, the small molecule SUMO inhibitors described hereinmay be used in methods for inhibiting a SUMOylation enzyme in a cell.Such methods may include a step of administering an effective amount ofa SUMO inhibitor compound to the cell.

According to the embodiments described herein, the SUMO inhibitorcompound is one or more of the SUMO inhibitors described herein. TheSUMO inhibitors may therefore be used in methods for inhibiting aSUMOylation enzyme (e.g., SUMO E1, SUMO E2 or any one or more of theSUMO E3 ligases) in a cell, as provided herein. In some embodiments,such methods for inhibiting a SUMOylation enzyme may include a step ofcontacting the cell with or administering to the cell, one or more SUMOinhibitors or a pharmaceutically acceptable derivative thereof,described herein.

The cell may be of any cell type that is associated with SUMOylation(e.g., a cell that overexpresses a SUMOylation enzyme, underexpresses aSUMOylation enzyme, or a cell that expresses a specific cellular proteinthat is SUMOylated in a disease or other physiological or pathologicalcondition). In certain embodiments, the cell type used in the methodsfor inhibiting a SUMOylation enzyme described herein may be a cell thatis part of a population of cells or a biological tissue that is present,in vivo, in a subject having the disease or other physiological orpathological condition. This may include a human or animal patient thatdevelops the disease or other physiological or pathological conditionor, alternatively, may include an animal or invertebrate model whereinthe disease, or physiological or pathological condition may be induced.In other embodiments, the cell type used in the methods for inhibiting aSUMOylation enzyme described herein may be a primary, secondary orimmortal cell line that is grown in culture. In certain aspects of thisembodiment, the method may be used in an in vitro or research setting toinvestigate the role of SUMOylation in the particular cell, disease, orcondition.

The term “effective amount” as used herein refers to an amount of acompound that produces a desired effect. For example, a population ofcells may be contacted with an effective amount of a compound to studyits effect in vitro (e.g., cell culture) or to produce a desiredtherapeutic effect ex vivo or in vitro. An effective amount of acompound may be used to produce a therapeutic effect in a subject, suchas preventing or treating a target condition, alleviating symptomsassociated with the condition, or producing a desired physiologicaleffect. In such a case, the effective amount of a compound is a“therapeutically effective amount,” “therapeutically effectiveconcentration” or “therapeutically effective dose.” The preciseeffective amount or therapeutically effective amount is an amount of thecomposition that will yield the most effective results in terms ofefficacy of treatment in a given subject or population of cells. Thisamount will vary depending upon a variety of factors, including but notlimited to the characteristics of the compound (including activity,pharmacokinetics, pharmacodynamics, and bioavailability), thephysiological condition of the subject (including age, sex, disease typeand stage, general physical condition, responsiveness to a given dosage,and type of medication) or cells, the nature of the pharmaceuticallyacceptable carrier or carriers in the formulation, and the route ofadministration. Further an effective or therapeutically effective amountmay vary depending on whether the compound is administered alone or incombination with another compound, drug, therapy or other therapeuticmethod or modality. One skilled in the clinical and pharmacological artswill be able to determine an effective amount or therapeuticallyeffective amount through routine experimentation, namely by monitoring acell's or subject's response to administration of a compound andadjusting the dosage accordingly. For additional guidance, seeRemington: The Science and Practice of Pharmacy, 21^(st) Edition, Univ.of Sciences in Philadelphia (USIP), Lippincott Williams & Wilkins,Philadelphia, Pa., 2005, which is hereby incorporated by reference as iffully set forth herein.

E1 contains several substrate-binding sites, including the ATP-bindingpocket (Lois & Lima 2005), two SUMO-binding surfaces (Lois & Lima 2005,Wang & Chen) and three Ubc9-binding surfaces (Wang et al. 2007; Wang &Chen; Huang et al. 2007). As discussed in the Examples below, thetricyclic SUMO inhibitors disclosed herein were found to competitivelyinhibit ATP binding. Since cellular concentrations of ATP are generallyhigh, this ATP inhibition by certain of the inhibitors described hereindoes not necessarily result in cell toxicity when administered to a cellor to a subject in an effective dose. However, inhibition of ATP bindingdoes sensitize cells to genotoxic stress and inhibit HIV infection asdiscussed further in the Examples below. Therefore, provided herein incertain embodiments described below are methods of using the tricyclicSUMO inhibitors disclosed herein to sensitize cells to genotoxic stressand/or to treat HIV and other viral infections (see FIG. 14).

In another embodiment, inhibition of ATP binding by the tricyclic SUMOE1 inhibitors disclosed herein makes the inhibitors useful as researchprobes for identifying and/or monitoring SUMOylation activity in vitro.In such embodiments, the tricyclic inhibitors may be conjugated to orotherwise associated with a label for use in various cellular assays.Labels that may be used in accordance with these embodiments mayinclude, but are not limited to, radiolabels such as the radionuclidesdescribed below and fluorophores, thiol-reactive labels, biotin andhapten derivatives, crosslinking and photoactivatable reagents, avidinsand lectins for use with antibodies, enzyme substrates and othersuitable fluorescent labels. For additional guidance, see MolecularProbes Handbook, A Guide to Fluorescent Probes and LabelingTechnologies, 11^(th) Edition, Invitrogen, 2010. (Iain Johnson andMichelle T. Z. Spence, Eds.), which is hereby incorporated by referenceas if fully set forth herein.

In such embodiments, the SUMO inhibitors may be used as probes in cellculture assays to determine the effect of SUMOylation activity in aparticular cell line. To test whether SUMOylation of a protein ofinterest in involved in a particular function, a labeled SUMO inhibitor(MLS-0207587) may be added to determine whether the output of the assaychanges. For example, a SUMO inhibitor probe was used to demonstratethat the inhibitor specifically inhibits recruitment of 53BP1 and BRCAto DNA damage sites (both of which are SUMO-dependent processes in theDNA damage response), thereby validating the role of SUMO inhibitors forsensitizing cancer cells to radiation (see FIGS. 15-19).

Methods for Treating Cancer

Provided herein in certain embodiments are methods for treating acondition or disease with one or more of the SUMO inhibitors (or“SUMOylation inhibitors” or “SUMOylation inhibitor compounds”) and thepharmaceutically acceptable derivative, solvates, salts andstereoisomers thereof, including mixtures thereof in all ratios,described herein These methods may include, but are not limited to,administering a therapeutically effective amount of the one or more SUMOinhibitors or pharmaceutically acceptable derivative, solvates, saltsand stereoisomers thereof, including mixtures thereof in all ratios to asubject having the condition or disease. In one embodiment, the SUMOinhibitor is a SUMO E1 inhibitor. In certain embodiments, the or one ormore SUMO inhibitors may be identified by methods described herein, forexample, using a high throughput screening method to identify a SUMOinhibitor followed by biochemical assays to confirm activities andcellular assays to validate the effects of such inhibitors in cells).

As used herein, the term “functionally effective derivative” or“pharmaceutically acceptable derivative” refers to any physiologicallyfunctional derivative of a novel SUMO inhibitor disclosed herein. Suchderivatives may include pharmaceutically acceptable salts or so-calledpro-drug-compounds, for example compounds according to the inventionthat are derivatized with alkyl groups, acyl groups, sugars or peptides,such as oligopeptides, that are easily degraded or metabolized to theactive compounds according to the invention. Such derivatives mayinclude biodegradable polymer derivatives of the compounds according tothe embodiments described herein. Suitable polymers and methods forproducing biodegradable polymeric derivatives are known in the art.Further, such derivatives include analogs that have substitutions ormodifications that one skilled in the art would recognize as having thesame, similar or improved function as the SUMO inhibitors describedherein. Upon administration to a subject, a functional derivative of aSUMO inhibitor is capable of providing, directly or indirectly, a SUMOinhibitor disclosed herein, an analog of a SUMO inhibitor disclosedherein or an active metabolite thereof. Such derivatives are clear tothose skilled in the art, without undue experimentation. For additionalguidance, see Burger's Medicinal Chemistry, Drug Discovery andDevelopment, 7th Edition, Wiley-Interscience, 2010, which isincorporated herein by reference as if fully set forth herein to theextent that it teaches physiologically functional derivatives.

As used herein, the term “solvate” refers to a complex of variablestoichiometry formed by a solute (in this invention, a SUMO inhibitorderivative comprising a structure described herein or a salt orphysiologically functional derivative thereof) and a solvent. Suchsolvents for the purpose of the invention may not interfere with thebiological activity of the solute. Examples of suitable solventsinclude, but are not limited to, water, aqueous solution (e.g. buffer),methanol, ethanol and acetic acid. Preferably, the solvent used is apharmaceutically acceptable solvent. Examples of suitablepharmaceutically acceptable solvents include, without limitation, water,aqueous solution (e.g. buffer), ethanol and acetic acid. Mostpreferably, the solvent used is water or aqueous solution (e.g. buffer).Examples for suitable solvates are the mono- or dihydrates oralcoholates of the compounds according to the invention.

Certain of the compounds described herein may contain one or more chiralatoms, or may otherwise be capable of existing as two or morestereoisomers, which are usually enantiomers and/or diastereomers.Accordingly, the compounds of this invention include mixtures ofstereoisomers, mixtures of enantiomers, as well as purifiedstereoisomers, purified enantiomers, or stereoisomerically enrichedmixtures, enantiomerically enriched mixtures. Also included within thescope of the invention are the individual isomers of the compoundsrepresented by the structures described herein as well as any wholly orpartially equilibrated mixtures thereof. The invention also covers theindividual isomers of the compounds represented by the structures aboveas mixtures with isomers thereof in which one or more chiral centers areinverted. Also, it is understood that all tautomers and mixtures oftautomers of the SUMO inhibitor compounds described herein are includedwithin the scope of the SUMO inhibitor compounds and preferably thestructures and scaffolds corresponding thereto.

Racemates obtained can be resolved into the isomers mechanically orchemically by methods known in the art. Diastereomers are preferablyformed from the racemic mixture by reaction with an optically activeresolving agent. Examples of suitable resolving agents are opticallyactive acids, such as the D and L forms of tartaric acid,diacetyltartaric acid, dibenzoyltartaric acid, mandelic acid, malicacid, lactic acid or the various optically active camphorsulfonic acids,such as camphorsulfonic acid. Also advantageous is enantiomer resolutionwith the aid of a column filled with an optically active resolving agent(for example dinitrobenzoyl phenylglycine); an example of a suitableeluent is a hexane/isopropanol/acetonitrile mixture. The diastereomerresolution mat also be carried out by standard purification processes,such as, for example, chromatography or fractional crystallization. Italso possible to obtain optically active SUMO inhibitor compounds by themethods described above by using starting materials which are alreadyoptically active.

SUMOylation inhibitors, when used for a short period of time, areunlikely to be toxic to normal (noncancerous) cells that divide slowly.Recent studies have shown that expression of a peptide inhibitor of thedown-stream effects of SUMOylation did not induce cytotoxicity in MCF-7(WT KRas status) cells in the absence of genotoxic stress (Li et al.2010).

The small molecule SUMO inhibitors and the pharmaceutically orfunctionally acceptable derivatives, solvates, salts and stereoisomersthereof, including mixture thereof in all ratios provided herein may beused to treat any condition or disease that is associated with alteredlevels of SUMOylation including, but not limited to, cancer, tumors,neoplastic conditions or syndromes, viral infections (e.g., HIV),cardiovascular disease, degenerative disease and genetic or hereditarydiseases. Such diseases may be associated with, for example, anoverexpression or underexpression of one or more SUMOylation enzymes orone or more specific proteins that are SUMOylated in the disease orcondition. Examples of specific proteins that may be SUMOylated in adisease or condition include, but are not limited to, p53, HDAC, cyclinsand other proteins in cancer; SOD1 in amyotrophic lateral sclerosis;ataxin-1 in spinocerebellar ataxia; huntingtin in Huntington's disease;tau, α-synuclein, DJ-1 or other proteins in Parkinson's disease; tau,APP or other proteins in Alzheimer's disease, lamin A in familialdilated cardiomyopathy; 1E1 and 1E2 in human CMV; and P6-Gag in HIV.

In one embodiment, the small molecule SUMO E1 inhibitors provided hereinare used to treat any cancer associated with increased or decreasedexpression of SUMO enzymes.

Cancers, tumors or other neoplastic conditions or syndromes that may betreated according to the embodiments described herein include, but arenot limited to adenoid cystic carcinoma, adrenal gland tumor,amyloidosis, anal cancer, appendix cancer, ataxia-telangiectasia,attenuated familial adenomatous polyposis, Beckwith-Wiedemann syndrome,bile duct cancer, Birt-Hogg-Dube syndrome, bladder cancer, bone cancer,brain tumor, breast cancer, carcinoid tumor, Carney Complex, cervicalcancer, childhood cancer (e.g., brain stem glioma, astrocytoma, centralnervous system, craniopharyngioma, Desmoplastic Infantile ganglioglioma,ependymoma, Ewings family of tumors, germ cell tumor, acutelymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin'slymphoma, Non-Hodgkin's lymphoma, medulloblastoma, neuroblastoma,osteosarcoma, pleuropulmonary blastoma, retinoblastoma,rhabdomyosarcoma, Wilms tumor), colorectal cancer, Cowden syndrome,endocrine tumor, endometrial cancer, esophageal cancer, eye cancer,eyelid cancer, fallopian tube cancer, familial adenomatous polyposis,familial malignant melanoma, gallbladder cancer, Gardner syndrome,gastrointestinal stromal tumor (GIST), gestational trophoblastic tumor,head and neck cancer, hereditary cancer (e.g., breast, ovarian, diffusegastric, leiomyomatosis, renal cell, mixed polyposis syndrome, non-VHLclear cell renal cell carcinoma, pancreatitis and papillary renal cellcarcinoma), HIV and AIDS-related cancer, islet cell tumor, juvenilepolyposis syndrome, kidney cancer, lacrimal gland tumor, laryngeal andhypopharyngeal cancer, leukemias (e.g., acute lymphocytic leukemia(ALL), acute myeloid leukemia (AML), B-cell leukemia, chroniclymphocytic leukemia (CLL), chronic myeloid leukemia (CML), eosinophilicleukemia, T-cell leukemia), Li-Fraumeni syndrome, liver cancer, lungcancer, Hodgkin's lymphoma, Non-Hodgkin's lymphoma, Lynch syndrome,mastocytosis, melanoma, meningioma, mesothelioma, Muir-Torre syndrome,multiple endocrine neoplasia types 1 and 2, multiple myeloma,myelodysplastic syndromes (MDS), MYH-associated polyposis, nasal cavityand paranasal sinus cancer, nasopharyngeal cancer, neuroendocrine tumor,neurofibromatosis types 1 and 2, nevoid basal cell carcinoma syndrome,oral and oropharyngeal cancer, ovarian cancer, pancreatic cancer,parathyroid cancer, penile cancer, Peutz-Jeghers syndrome, pituitarygland tumor, prostate cancer salivary gland cancer, rhabdomyosarcoma,sarcoma, skin cancer (non-melanoma), small bowel cancer, stomach cancer,testicular cancer, thymoma, thyroid cancer, tuberous sclerosis syndrome,Turcot syndrome, unknown primary cancer, uterine cancer, vaginal cancer,Von Hippel-Lindau syndrome, vulvar cancer, Waldenstrom'smacroglobulinemia, Werner syndrome, and xeroderma pigmentosa.

In some embodiments, cancers, tumors or other neoplastic conditions orsyndromes that may be treated according to the embodiments describedherein include, but are not limited to those that are considered to beresponsive to radiation therapy (alone or in combination with one ormore other treatments), including, but not limited to, brain cancer,breast cancer, cervical cancer, colorectal cancer, endometrial cancer,head and neck cancer, Hodgkin's disease and local extranodal lymphoma,melanoma, ovarian cancer, prostate cancer, rhabdomyosarcoma,retinoplastoma, skin and lip cancer, soft tissue carcinoma, testicularcancer, thyroid cancer, and Wilms tumor.

In some embodiments, the SUMO inhibitor is part of a pharmaceuticalcomposition. The pharmaceutical composition may include one or more SUMOinhibitor and a pharmaceutically acceptable carrier. In someembodiments, the pharmaceutical composition/SUMO inhibitor may beadministered in combination with one or more DNA-damaging therapies. Inthis case, the SUMO inhibitor may sensitize the target cells/cancercells to the DNA-damaging therapy. Thus, the DNA-damaging therapy ismore effective, and allows the use of lower doses, thereby minimizing oreliminating harm to healthy cells.

The term “treat,” “treating” or “treatment” as used herein with regardto a condition or disease may refer to preventing a condition ordisease, slowing the onset or rate of development of the condition ordisease, reducing the risk of developing the condition or disease,preventing or delaying the development of symptoms associated with thecondition or disease, reducing or ending symptoms associated with thecondition or disease, generating a complete or partial regression of thecondition or disease, or some combination thereof.

A “pharmaceutically acceptable carrier” may refer to a pharmaceuticallyacceptable material, composition, or vehicle that is involved incarrying or transporting a compound of interest from one tissue, organ,or portion of the body to another tissue, organ, or portion of the body.For example, the carrier may be a liquid or solid filler, diluent,excipient, solvent, or encapsulating material, or some combinationthereof, described in further detail below. Each component of thecarrier must be “pharmaceutically acceptable” in that it must becompatible with the other ingredients of the formulation. It also mustbe suitable for contact with any tissue, organ, or portion of the bodythat it may encounter, meaning that it must not carry a risk oftoxicity, irritation, allergic response, immunogenicity, or any othercomplication that excessively outweighs its therapeutic benefits.

Pharmaceutically acceptable carriers are well known in the art andinclude, for example, aqueous solutions such as water or physiologicallybuffered saline or other solvents or vehicles such as glycols, glycerol,oils such as olive oil or injectable organic esters, or emulsions suchas oil/water emulsions or a triglyceride emulsion, various types ofwetting agents, tablets, coated tablets and capsules. A suitablepharmaceutically acceptable carrier may be selected taking into accountthe chosen mode of administration.

A pharmaceutically acceptable carrier can also contain physiologicallyacceptable compounds that act, for example, to stabilize or to increasethe absorption of the conjugate. Such physiologically acceptablecompounds include, for example, carbohydrates, such as glucose, sucroseor dextrans, antioxidants, such as ascorbic acid or glutathione,chelating agents, low molecular weight proteins or other stabilizers orexcipients. One skilled in the art will know that the choice of apharmaceutically acceptable carrier, including a physiologicallyacceptable compound, depends, for example, on the route ofadministration of the composition.

In one preferred embodiment, the pharmaceutical carrier may be a liquidand the pharmaceutical composition would be in the form of a solution.In another equally preferred embodiment, the pharmaceutically acceptablecarrier is a solid and the pharmaceutical composition is in the form ofa powder or tablet.

A solid carrier can include one or more substances which may also act asflavoring agents, lubricants, solubilizers, suspending agents, fillers,glidants, compression aids, binders or table-disintegrating agents, itcan also be an encapsulating material. In powders, the carrier is afinely divided solid that is in admixture with the finely divided activeingredient. In tablets, the active-ingredient is mixed with a carrierhaving the necessary compression properties in suitable proportions andcompacted in the shape and size desired. The powders and tablets maycontain up to 99% of the active ingredient. Suitable solid carriersinclude, for example, calcium phosphate, magnesium stearate, talc,sugars, lactose, dextrin, starch, gelatin, cellulose,polyvinylpyrrolidine, low melting waxes and ion exchange resins.

Besides containing an effective amount of the SUMO inhibitors describedherein the pharmaceutical compositions may also include suitablediluents, preservatives, solubilizers, emulsifiers, adjuvant and/orcarriers.

The compound can be administered in the form of a sterile solution orsuspension containing other solutes or suspending agents, for example,enough saline or glucose to make the solution isotonic, bile salts,acacia, gelatin, sorbitan monoleate, polysorbate 80 (oleate esters ofsorbitol and its anhydrides copolymerized with ethylene oxide) and thelike.

Optimal dosages to be administered may be determined by those skilled inthe art, and will vary with the particular compound in use, the severityof the disease state, drug combination(s), reaction sensitivities, andresponse to therapy. Additional factors depending on the particularsubject being treated, including the general health of the subject, theage, weight, gender and diet of the subject, and time and frequency ofadministration, will result in a need to adjust dosages. Administrationof the SUMO inhibitors or pharmaceutical composition thereof may beeffected continuously or intermittently. In any treatment regimen, theSUMO inhibitors or pharmaceutical composition may be administered to apatient either singly or in a cocktail containing other therapeuticagents, compositions, or the like, including, but not limited to,tolerance-inducing agents, potentiators and side-effect relievingagents. Preferred potentiators include monensin, ammonium chloride,perhexyline, verapamil, amantadine, and chloroquine. All of these agentsare administered in generally-accepted efficacious dose ranges such asthose disclosed in the Physician's Desk Reference, 41st Ed., PublisherEdward R. Barnhart, N.J. (1987), which is incorporated herein byreference.

The term “subject” may refer to a human or any other animal, animalmodel or invertebrate model having a condition, a disease, a cell, or apopulation of cells that may be treated or used accordance with themethods or with the compounds or compositions described herein. In oneembodiment, the subject is a human subject having a disease orcondition, such as those described herein. In other embodiments, thesubject is any other animal having such a disease or condition,including an animal model used as a research tool that is developed tohave the disease or condition or has one or more aspects, attributes,symptoms, or other variables associated with the disease or condition.As such, the SUMO inhibitors described herein may be used as researchtools. Such animals or animal models may include, but are not limitedto, mice, rats, rabbits, monkeys, pigs, dogs, cats, and birds. Inanother embodiment the subject may be any other vertebrate orinvertebrate model that can be used as a research tool including, butnot limited to, a fish (e.g., zebrafish), an insect (e.g., drosophila),nematode (e.g., c. elegans), mollusk (e.g., aplesia californicus).

In some embodiments, a cell or population of cells grown in culture maybe used in accordance with the methods or with the compounds orcompositions described herein. The cell or population of cells may bederived from or cultured from one or more subjects described above, andmay used as a research tool in accordance with the embodiments describedherein.

Administering one or more compounds or compositions described herein tothe subject, cell or population of cells to investigate one or moremechanisms or other aspects of a condition or disease described herein;or for investigating the effect of one or more compounds or compositionsdescribed herein when administered to the cell, population of cells orsubject.

The term “route of administration” or “administering” may refer to anyadministration pathway known in the art, including but not limited toaerosol, enteral, nasal, ophthalmic, oral, parenteral, rectal,transdermal (e.g., topical cream or ointment, patch), or vaginal.“Transdermal” administration may be accomplished using a topical creamor ointment or by means of a transdermal patch. “Parenteral” refers to aroute of administration that is generally associated with injection,including infraorbital, infusion, intraarterial, intracapsular,intracardiac, intradermal, intramuscular, intraperitoneal,intrapulmonary, intraspinal, intrasternal, intrathecal, intrauterine,intravenous, subarachnoid, subcapsular, subcutaneous, transmucosal, ortranstracheal.

The tricyclic SUMO E1 inhibitors characterized herein were not toxic tohuman hepatocytes at concentrations up to 50 μM (FIG. 9), but inducedsignificant sensitivity to radiation in various cancer cell lines withan LC₅₀ of approximately 10 μM or less (see FIG. 8). These findings areparticularly important in developing treatment strategies and regimensfor increasing the efficacy of radiation therapy, because radiationtherapy is delivered locally to tumors, but systemically deliveredSUMOylation inhibitors should not cause significant damage tonon-irradiated normal cells. Additionally, such inhibitors may bedirectly applied to rectal cancers locally, an established strategy.Taken together, the studies described herein are significant in thatthey should lead to development of new paradigms of more effective CRTthat are applicable to a wide range of cancers, as well as the firstsmall molecular probes of SUMOylation to elucidate its role in cellularregulation.

Therefore, according to some embodiments, a SUMO inhibitor, alone or aspart of a pharmaceutical composition, may be administered in combinationwith one or more additional therapeutic agents to treat a condition ordisease that is associated with altered levels of SUMOylation. In someembodiments, the one or more additional therapeutic agents include oneor more DNA-damaging (or “genotoxic”) therapy. Administration of theSUMO inhibitor in combination with the one or more genotoxic therapy mayincrease the efficacy of the one or more additional therapeutic agents,produce a synergistic effect between the inhibitor and the one or moreadditional therapeutic agents, sensitize cells affected by the conditionor disease associated with altered levels of SUMOylation, or acombination thereof.

The term “in combination” or “in combination with” as used herein, meansin the course of treating the same disease or condition in a subjectusing two or more therapies (e.g., agents, drugs, treatment regimens,treatment modalities or a combination thereof) in any order. Thisincludes simultaneous administration (or “co-administration”),administration of a first therapy prior to or after administration of asecond therapy, as well as in a temporally spaced order of up to severaldays apart. Such combination treatment may also include more than asingle administration of any one or more therapies. Further, theadministration of the two or more therapies may be by the same ordifferent routes of administration.

According to the embodiments described herein, genotoxic therapies thatmay be administered in combination with the SUMO inhibitors to treat adisease or condition associated with SUMOylation may include, but arenot limited to, administration of one or more radiation therapyregimens, administration of one or more DNA-damaging or genotoxicchemotherapeutic agents, or a combination thereof.

Administration of one or more radiation therapy regimens may include anysource of ionizing radiation, including x-rays, gamma-rays, alphaparticles, beta particles or a combination thereof. These radiationsources may be administered using any sealed source (e.g., external beamradiation therapy, brachytherapy, stereotactic radiation, virtualsimulation, 3-dimensional conformal radiation therapy (3DCRT), andintensity modulated radiation therapy (IMRT), image guided radiationtherapy (IGRT), particle therapy) or unsealed source (e.g., systemicradioisotope therapy). Examples of radiation sources or imaging methodsthat may be used according to the embodiments described herein mayinclude, but are not limited to, radiographs, computed tomography (CT),fluoroscopy, positron emission tomography (PET), single photon emissioncomputed tomography (SPECT), radionuclides used alone or with an imagingmethod such as CT, PET or SPECT (e.g., Barium-133, Cadmium-109,Cobalt-57, Cobalt-60, Iodine-131, Iodine-131methaiodobenzylguanidine(MIBG), Europium-152, Manganese-54, Sodium-22, Zinc-65, Technetium-99m,Polonium-210, Strontium-90, Thallium-204, Carbon-14, Lutetium-177,Yttrium-90, Phosphorus-32, Strontium-89, Samarium-153, Radium-223,Bismuth-213), radioimmunotherapy (e.g., Yttrium (⁹⁰Y) ibritumomabtiuxetan, Iodine (¹³¹I) tositumomab)

In some embodiments, the one or more genotoxic chemotherapeutic agentsthat may be administered in combination with the SUMO inhibitorsdescribed herein include, but are not limited to, (i) alkylating agents,platinum analogues or other alkylating-like or nonclassical alkylatingagents (e.g., carmustine, streptozocin, busulfan, chlorambucil,ifosfamide, cyclophosphamide, thiotepa, lomustine, cisplatin,carboplatin, mechlorethamine, chloambucil, oxaliplatin, uramustine,melphalan, nedaplatin, satraplatin, triplatin tetranitrate,procarbazine, altretamine and temozolomide); (ii) intercalating agents(e.g., doxorubicin, epirubicin, danorubicin, daunomycin, proflavine,ethidium bromide, berberine, thalidomide and dactinomysin); (iii)topoisomerase inhibitors (etopocide, topotecan, irinotecan, amsacrine.camptothecin, lamellarin D, teniposide, aurintricarboxylic acid andHU-331); and (iv) cytotoxic antibiotics (e.g., actinomycin. valrubicin,idarubicin, bleomycin, plicamycin and mitomysin).

Having described the invention with reference to the embodiments andillustrative examples, those in the art may appreciate modifications tothe invention as described and illustrated that do not depart from thespirit and scope of the invention as disclosed in the specification. Theexamples are set forth to aid in understanding the invention but are notintended to, and should not be construed to limit its scope in any way.The examples do not include detailed descriptions of conventionalmethods. Such methods are well known to those of ordinary skill in theart and are described in numerous publications. Further, all referencescited above and in the examples below are hereby incorporated byreference in their entirety, as if fully set forth herein.

Example 1 SUMO-1, -2, and -3 Expression in Colorectal Cell Lines

SUMO1, -2, and -3 mRNA levels were measured in the colorectal cancercell lines HCT116 and HT29, which represent the majority of colorectalcancer types (e.g., they have chromosome instability or are mismatchrepair deficient). Specifically, gene expression levels were measuredfor both SUMO E1 subunits (SAE1 and SAE2); SUMO E2 (Ubc9); the SUMO E3ligases PIAS1, PIAS2, PIAS3, PIAS4, RanBP2, MMS21; and de-SUMOylationenzymes SENP1, 2, 3, 4, 5, 6, and 7. Expression levels in HCT116 andHT29 were compared to those in normal colon mucosa. GAPDH expressionlevels were used as a control.

Significantly higher mRNA levels were observed for mostSUMOylation-related genes (Table 2). The two E1 subunits (SAE1 and SAE2)were the most elevated, with significantly greater increases inexpression than Ubc9 (E2) and PIAS3 (E3). This is significant becauseUbc9 and PIAS3 were both previously found to be elevated in many cancertypes (Wang 2004; Mo 2005).

TABLE 2 SUMO-1, -2, and -3 expression in colorectal cell lines Fold-Fold- Avg. change in change in HCT116 HT29 control HCT116 vs. HT29 vs.Gene (RPKM*) (RPKM*) (RPKM*) control control E1 103.75 108.24 17.34 5.986.24 (SAE1) E2 60.09 91.02 6.13 9.80 14.85 (SAE2) E3 6.64 7.95 1.53 4.345.20 (PIAS3) E2 113.94 101.28 29.31 3.89 3.46 (Ubc9) GAPDH 2100.402313.06 1094.17 1.92 2.11 RPKM: reads per kilobase per million mapped

Immunohistochemistry studies were performed on stage II and IIIcolorectal tumor specimens and matched normal tissues. Consistent withthe mRNA expression results, SAE1 and SAE2 were the most significantlyoverexpressed SUMOylation-related proteins in tumor specimens relativeto the matched normal tissues (Wiatrek et al. Differential expression ofsmall ubiquitin-like modifier family of proteins in patients withcolorectal adenocarcinoma; ASCO Abstract, 2011, which is herebyincorporated by reference, as if fully set forth herein; seehttp://www.asco.org/ascov2/Meetings/Abstracts?&vmview=abst_detail_view&confide=103&abstractID=71189).

SAE1 and SAE2 levels were found to be the only SUMO proteins withincreased expression in resistant tumors after CRT in comparison topretreatment biopsy samples. To validate this clinical observation, aradioresistant HCT116 line was developed by irradiating (2Gy/day) amouse xenograft tumor for one week, cutting out the xenograft and usingit to start a primary culture, then irradiating the culture (2Gy/day)for another week. Comparison of the radioresistant HCT116 line with theparental line showed that the SAE2 level was approximately 3-foldgreater in the radioresistant line. The level of other SUMO enzymes didnot change significantly. The results of these studies indicate thatupregulated SUMO E1 levels are correlated with a patient's response toCRT, and that the E1 level increases after CRT in resistant tumors.

Example 2 Development of Tricyclic SUMO E1 Inhibitors

Through the Molecular Library Probe Production Center Network (MLPCN),at least 300,000 compounds were screened using a TR-FRET method, anALPHASCREEN™ method or both, and tested for their ability to inhibitSUMOylation of a target protein via SUMO E1 or SUMO E2. The assays werebased on SUMOylation of the target protein RanGAP1, which is a proteinthat is efficiently SUMOylated with only the SUMO E1 and E2 enzymes, anddoes not use E3 ligases. A fluorescence resonance energy transfer (FRET)assay was the primary assay followed by a chemoluminescence-basedsecondary assay using ALPHA screen to eliminate false positive hits.Then, the hits were screened by a poly-ubiquitination assay usingubiquitin, ubiquitin E1, Ubc5 and Apc11 to eliminate inhibitors notspecific to SUMOylation. The screening identified a potent family ofSUMOylation inhibitors based on a tricyclic scaffold.

Approximately 200 small molecule inhibitors of SUMO E1 were identifiedaccording to these methods. These inhibitors can be classified into tencompound scaffolds, three of which were selected for furthercharacterization based on having a higher potency as compared to othermolecule inhibitors that were identified. Exemplar studies to determinethe efficacy and potency of the inhibitors are illustrated in FIGS.10-13. Several SUMO E1 inhibitors based on these scaffolds weresynthesized, purchased, or both for further characterization (FIGS.20A-C)

One of the selected scaffolds is a tricyclic scaffold containing the1H-pyrrolo-[2,3-b]quinoxalines (FIG. 3A). Approximately 30 compoundswith this scaffold were screened for their ability to inhibit SUMO E1.The structures of twelve of these compounds are set forth in FIG. 4 andTable 1 above, along with the in vitro IC₅₀ of each compound for SUMOE1. Table 1 shows representative compounds of the SUMOylation inhibitorsderived from the singleton scaffold. Of the screened compounds,quinoxaline 1 (FIG. 3B) was identified as one of the most potent SUMO E1inhibitor identified and selected as a lead compound (identified hereinas MLS0437113), with an in vitro IC₅₀ of approximately 0.25-0.5 μM and aKd of approximately 180 nM (FIG. 5, upper panel and FIG. 6). Inaddition, quinoxaline 1 is more than 100-fold more selective forSUMOylation than for the homologous ubiquitin E1, suggesting that itdoes not inhibit SUMO E1 through non-specific mechanisms (FIG. 5).Another compound from this scaffold group, quinoxaline 2 (FIG. 3B), doesnot inhibit SUMO E1. Quinoxaline 2 is structurally very similar toquinoxaline 1, but lacks a phenyl group. This suggests that this phenylgroup is important to SUMO E1 inhibition. Since quinoxaline 2 did notexhibit SUMO E1 inhibition, it was used as a control for furtherstudies.

Example 3 Synthesis of Tricyclic SUMO E1 Inhibitors

The tricyclic SUMOylation inhibitor compounds described herein may besynthesized using any suitable method known in the art, includingaccording to the representative protocols shown below.

Protocol 1: Generation of Compound D According to Scheme 1.

2-tosylacetonitrile B (0.108 g, 0.5 mmol) was transferred to a 250 mlround-bottom flask and dissolved in 10 ml DMSO. Cesium carbonate (0.245g, 0.75 mmol) was added to the reaction mixture followed by addition of2,3-dichloroquinoxaline (0.1 g, 0.5 mmol). The reaction mixture wasstirred at room temperature overnight. The crude reaction mixture wasdiluted with 50 ml ethyl acetate and washed with 1N HCl (2×50 mL) andbrine solution (2×50 mL). The organic layer was collected and thesolvent was removed under reduced pressure to yield the crude product C(2-(3-chloroquinoxalin-2-yl)-2-tosylacetonitrile) which was taken to thenext step without further purification. Compound C (0.150 g, 0.42 mmol)was transferred to a microwave vessel containing 1 ml butyl amine. Thereaction mixture was subjected to microwave irradiation at 70° C. for 30min. The resultant crude reaction mixture was purified using columnchromatography to yield compound D as shown in Scheme 1 (0.105 g, 64%yield). ¹H NMR (500 MHz, Chloroform-d) δ 8.24-8.15 (m, 3H), 7.93 (dd,J=8.2, 1.5 Hz, 1H), 7.58 (dddd, J=25.1, 8.3, 6.9, 1.6 Hz, 2H), 7.29 (d,J=8.1 Hz, 2H), 6.57 (bs, 2H), 4.19 (t, J=7.4 Hz, 2H), 2.38 (s, 3H),1.86-1.73 (m, 2H), 1.48-1.38 (m, 2H), 0.99 (t, J=7.3 Hz, 3H). ESI (m/z):395 (M+H⁺).

Protocol 1: Generation of Compound E According to Scheme 2.

Compound D (250.0 mg, 0.633 mmol) generated in Scheme 1 was dissolved in5 ml dry THF at 0° C. followed by addition of NaH (22.0 mg, 0.951 mmol).The resulting reaction mixture was stirred for 5 minutes followed byaddition of benzoyl chloride (89.0 mg, 0.633 mmol). The progress of thereaction was monitored using TLC and after completion of reaction (˜30min) the reaction mixture was quenched by addition of NH₄Cl solution.The crude reaction mixture was diluted with 50 ml ethyl acetate andwashed with 1N HCL (2×50 mL) and brine solution (2×50 mL). The organiclayer was collected and concentrated under reduced pressure to yield thecrude product which was purified using column chromatography to furnishcompound E as shown in Scheme 2 (145.0 mg, 46.0% yield). ¹H NMR (500MHz, Chloroform-d) δ 10.48 (s, 1H), 8.40-8.28 (m, 1H), 8.23-8.14 (m,2H), 8.15-8.07 (m, 3H), 7.79-7.70 (m, 3H), 7.66 (t, J=7.6 Hz, 2H), 7.25(d, J=8.2 Hz, 2H), 4.59 (t, J=7.4 Hz, 2H), 2.35 (s, 3H), 1.95-1.79 (m,2H), 1.34-1.23 (m, 2H), 0.91 (t, J=7.4 Hz, 3H). ESI (m/z): 500 (M+H⁺).

Protocol 2: Generation of Compound D According to Scheme 3.

Malononitrile B (0.66 g, 10 mmol) was dissolved in 20 ml DMSO. Cesiumcarbonate (4.87 g, 15 mmol) was added to the reaction mixture followedby addition of 2,3-dichloroquinoxaline (2.0 g, 10 mmol). The reactionmixture was stirred at room temperature overnight. The crude reactionmixture was diluted with 50 ml ethyl acetate and washed with 1N HCl(2×50 mL). the mixture was extracted with ethyl acetate (2×80 mL). Thecombined organic layer was washed with brine solution (2×50 mL). Theorganic layer was collected and the solvent was removed under reducedpressure to yield the crude product C2-(3-chloroquinoxalin-2-yl)malononitrile (2.0 g) which was used in thenext step without further purification. Compound C (0.22 g, 1 mmol) wastransferred to a microwave vessel containing 1 ml butyl amine. Thereaction mixture was subjected to microwave irradiation at 70° C. for 30min. The resultant crude reaction mixture was purified using columnchromatography to yield compound D as shown in Scheme 3 (0.20 g, 86%yield). ESI (m/z): 266 (M+H⁺).

Protocol 2: Generation of Compound E According to Scheme 4.

Compound D (200.0 mg, 0.76 mmol) was dissolved in 9 ml dry THF at 0° C.under nitrogen atmosphere followed by addition of NaH (27.0 mg, 1.13mmol). The resulting reaction mixture was stirred for 5 minutes followedby addition of acetyl chloride (107.0 mg, 0.76 mmol). The progress ofthe reaction was carefully monitored using TLC and after completion ofreaction (−30 min) the reaction mixture was quenched by addition ofNH₄Cl solution (30 mL). The crude reaction mixture was extracted withethyl acetate (3×30 mL). The combined organic layer was washed withbrine solution (50 mL). The organic layer was dried over anhydroussodium sulfate, filtered and concentrated under reduced pressure toyield the crude product which was purified using column chromatographyto furnish compound E as shown in Scheme 4 (170.0 mg, 61% yield) asyellow solid. ESI (m/z): 370 (M+H⁺).

An additional synthesis strategy was developed for generating tricyclicscaffold derivatives, including quinoxaline. The synthesis route forquinoxaline 1 is set forth in Scheme 5.

Briefly, upon heating commercially available nitrile 3 and2,3-dichloroquinoxaline (4) an excellent yield of monosubstitutedquinoxaline derivative 5 was obtained. This reaction can be carried outon a multigram scale. Treatment of 5 with n-butylamine for 3 days led todisplacement of chloride and cyclization, which afforded quinoxaline 2in moderate yields. Sulfonylation of quinoxaline 2 gave quinoxaline 1.

The roles of R₁, R₂, and R₃ on the quinoxaline core may also beevaluated by introducing different substituents. This can be readilydone at different points in the synthesis. Potential substitutionsinclude various stabilized carbonanions in step 1, different alkyl andaryl amines in step 2, and different acylating groups (includingsulfonyl and phosphoryl) in step 3.

Alternatively, to evaluate the importance of the large quinoxalinetemplate, smaller analogs such as2,3-dichloro-2,3-pyrazinedicarbonitrile (7) in place of quinoxaline 4may be prepared and evaluated. Following the chemistry outlined inScheme 6, this may lead to pyrrolo[2,3-b]pyrazine analog 8 for directcomparison with quinoxaline analog 1. The use of dichloropyrazine 7 hasrecently been shown to undergo monosubstitution with carbanionicnucleophiles (Chekmarev 2006).

It is likely that the benzene moiety of the quinoxaline ring system isimportant for binding and specificity. To investigate the role of thisstructural unit, the benzene ring may be derivatized with varioushydrophobic and hydrogen bond donor/acceptor groups as represented by R₄(Scheme 7). This synthetic route is amenable for the proposedmodifications. Modifications may be introduced at the front end of thesynthetic scheme, since many mono-, di- and tri-substitutedo-phenylenediamines 9 are readily available either through commercialsources or known procedures (Ohmori 1997).

The effects of substituting the sulfone moiety can be evaluated byintroducing an amide group during the initial aryl substitution reactionby converting dichlororquinoxaline 4 to intermediate 12 usingcyanoacetonitrile (Scheme 8). Cyclization with amine and acylation orsulfonylation would produce a nitro derivative 15. Reduction of thenitro group followed by acylation or sulfonylation would givefunctionally substituted quinoxaline 16.

Example 4 Evaluation of the Analogues and Identification of AdditionalLead Compounds

SUMOylation inhibitor analogues described herein may be tested usingvarious biochemical assays, as described in the Examples herein, toexamine their efficacy and selectivity for SUMOylation. In addition, NMRstudies described in FIG. 6 and elsewhere herein, may be carried out inconjunction to investigate changes of the binding mode on the E1. SUMOE1 specific inhibitors with more potent enzymatic inhibitory effect thancompound 1 or improved stability may be selected based on potency (e.g.,K_(d) or IC₅₀ values) for further in vivo testing. in vivo testing willstart with Tricyclic compound 1 described herein.

Investigation of the Structural Mechanism of Inhibition.

As discussed above, tricyclic compounds described herein bind andcompete for the ATP-binding pocket. Using the binding epitope of theinhibitor derived from NMR saturation transfer difference (STD) studies(FIG. 6), the program HADDOCK (de Vries et al. 2010; van Dijk et al.2005; Dominguez et al. 2003) may be used to determine whether saidinhibitor can fit into the ATP-binding pocket based on guided dockingcalculations of the protein-inhibitor complex. Further, previouslydescribed methods (Tatham et al. 2003; Wang & Chen 2010; Wang et al.2010; Want et al. 2009; Wang et al. 2007; Thatham et al. 2005; Tatham etal. 2003a; Liu et al. 2002; Liu et al. 1999) may be used to determinewhether the inhibitor binds to an allosteric site instead of or inaddition to directly competing with ATP. A structural allosteric effectwould likely affect the binding of other substrates (SUMO and Ubc9) inaddition to or instead of ATP.

Various forms of the E1 have been crystallized, including the freeenzyme, E1-ATP complex, E1-ATP-SUMO complex, E1 in complex with an ATPanalogue, and E1 in complex with a SUMO-adenylate mimic (Lois & Lima2005, Olsen et al. 2010), therefore, existing crystallization conditionsmay be used initially to obtain crystals. Then, the inhibitors may besoaked into the crystals. Additionally, the E1-inhibitor complex may beco-crystallized using one or more conditions identified by acrystallization robot that can screen for conditions that allowcrystallization of a protein. Alternatively, Nuclear Overhauser Effects(NOEs) between an inhibitor and SUMO E1 may be used to obtain theintermolecular distances between the protein and the inhibitor insolution, as previously described (Pellecchia et al. 2002). In additionto Met-methyl groups, the methyl groups of Ile, Leu and Val as may belabeled as previously described (Religa & Kay 2010). The intermoleculardistance constraints may then be used to determine structuralcalculations of the protein-inhibitor complexes, as described previously(Wang et al. 2007). NMR linewidths of the bound ligands and proteinsidechains at the binding interface also provide information on thedynamics at the binding pocket.

Accurate estimation of binding affinities of protein-ligand complexes isstill a challenge in the computational field. Therefore, medicinalchemistry efforts may be used in parallel with structural studies andrational design. The structural insights on the ligand-binding pocket ofE1 may be incorporated into the lead compound design. For example,identification of hydrogen-bonding donors or acceptors in the pocket arematched by incorporating complimentary hydrogen-bond acceptor or donorgroups in the ligand. The side of the ligand that does not form directcontacts with the enzyme is then derivatized to improve pharmacokineticand pharmacodynamic properties.

The medicinal chemistry methods described herein in combination withbinding mode and inhibition analysis provides structure-activityrelationship (SAR) information and an understanding of how the SUMO E1enzyme is inhibited by small molecules. In addition, the medicinalchemistry effort may identify additional inhibitors with higher potencyand/or higher in vivo stability than those already described. Given theimportant roles of SUMOylation in cancer, development ofneurodegenerative diseases and viral infection (Sarge et al. 2009), aswell as the lack of any inhibitors targeting SUMOylation enzymes, thesmall molecular inhibitors of SUMO E1 described herein should have asignificant impact on the treatment of these diseases as well as inbiomedical research for determining the pathogenic pathways involved inthe development of these life-threatening diseases. Lead compounds maybe used for preclinical and early phase clinical studies to examine howSUMOylation can be targeted for the development of innovative therapiesfor these life-threatening diseases.

In some aspects, an inhibitor may bind to more than one surface of theSUMO E1, as evidenced by NMR line-width changes of the inhibitors withaddition of the SUMO E1 (Reibarkh et al. 2006). In such cases, structuredetermination may be carried out under the appropriate inhibitor: E1stoichiometry.

Example 5 NMR Characterization of Tricyclic SUMO E1 Inhibitors

NMR saturation transfer difference (STD) experiments showed thatquinoxaline 1 bound E1 in a reversible manner and competed with thebinding of ATP. Using 80 μM ATP and 2 μM E1 in the presence of 0.4 mMMg²⁺, the STD of ATP was observed (FIG. 6A). Addition of 30 μMquinoxaline 1 to the above sample resulted in a reduction in the STD ofATP by more than 60% and observation of a significant STD effect of theinhibitor, indicating that the inhibitor competes efficiently with ATPwith less than half the concentration of ATP. Following these results, amore detailed competitive STD experiment was conducted (FIG. 6B).Previous studies indicate that the K_(d) of ATP binding to E1 in thepresence of Mg²⁺ is approximately 0.2 μM (Tokgoz 2006), and thus basedon the competitive STD data (FIG. 6B) the inhibitor-enzyme dissociationconstant (K_(d)) was estimated to be approximately 180 nM using theestablished method (Mayer 2001).

Example 6 Inhibition of SUMOylation by Tricyclic Inhibitors

To investigate whether quinoxaline 1 specifically inhibits SUMO E1 incells, the structurally similar inactive analog (quinoxaline 2) was usedas a control. Quinoxaline 1 or 2 was added to cell culture media atvarious concentrations for 18 hours, after which SUMOylated proteinswere detected using anti-SUMO antibodies. Quinoxaline 1 inhibitedSUMOylation in a dose-dependent manner in colorectal cell lines (HCT116and HT29) as well as other cancer cell lines (glioblastoma U373) (FIG.7). Importantly, quinoxaline 2 did not exhibit any inhibitory effectseven at 16 μM, a concentration at which quinoxaline 1 inhibited globalSUMOylation by more than 80 percent. Thus, quinoxaline 1 has potent andspecific inhibitory effects in various cancer cell lines.

Example 7 Sensitization of Cancer Cells to Genotoxic Stress

Quinoxaline 1 was added to cultures of various cancer cell lines for 4hours, after which cells were irradiated at 4 Gy. Forty-eight hoursafter irradiation, cell viability was measured by MTS assay. Addition ofthe inhibitor significantly enhanced the sensitivity of tested cancercell lines to radiation (FIG. 8). The inhibitor also induced sometoxicity in selected cancer cell lines at a lower IC₅₀ than that whichcaused toxicity in immortalized human hepatocytes Fa2N-4 (no toxicitywas detected with concentrations up to 50 μM; FIG. 9). Quinoxaline 2 didnot cause toxicity or demonstrated sensitization effects in any of thetested cell lines, consistent with its lack of inhibitory effect toSUMOylation.

Evaluate SUMOylation Inhibitors in Cancer Cell Lines.

Because the SUMO E1 enzyme is more crucial to survival of cells withcertain genetic backgrounds (Luo et al. 2009), lead compound 1 may besubmitted for screening of a panel of 60 cancer cell lines at NIH toidentify other genetic backgrounds in which the SUMO E1 is more criticalto viability. The results may be further validated by siRNA knockdownand molecular biological studies.

The extent to which the SUMOylation inhibitors can enhance cancer cellsensitivity to DNA-damaging chemotherapeutic drugs, includingdoxorubicin, 5-fluorouracil, camptosar and eloxatin, may be determinedand is compared to that of chemotherapeutic drugs that do not induce DNAdamage in colorectal cancer cell lines HT-29, HCT-116 and DLD1.Compounds with in vitro efficacy similar to or greater than that ofcompound 1 may be assessed for their effects on sensitizing colorectalcancer cells to radiation, as described in FIG. 8 (MTS assay).Additional studies may be carried out with different combination ofchemotherapeutic agents with radiation and with and without SUMO E1inhibitor. In addition, using compound 1, and inhibitors developed usingthe methods described in the Examples above, more sensitive cytotoxicityassays may be carried out using DIMSCAN, a semiautomaticfluorescence-based digital image microscopy system that quantifiesrelative total viable cell numbers in tissue culture with highsensitivity and linearity over a range >4 logs of cytotoxicityassessment (Keshelava et al. 2005). Clonogenic assays may be performedto examine long-term survival, as previously described (Li et al. 2010).The toxicity of the lead inhibitors identified in the Examples above tonormal cells may be tested using immortalized human hepatocytes (Fa2N-4cells) to ensure that the lead compounds do not have high toxicity tonormal cells.

Evaluation of the Molecular Mechanism of the Inhibitors.

To further elucidate the role of the SUMOylation inhibitors insensitizing cancer cells to ionizing radiation, compound 1 (FIG. 3) andadditional lead compounds identified according to the methods describedabove may be analyzed to determine how they affect early events in theDNA damage response, such as phosphorylation of histone H2AX and thekinases ATM, ATR and DNA-PKcs, and recruitment of 53BP1 and BRCA1 to DNAdamage sites that are critical for DNA DSB repair. Further, themechanism of how SUMOylation inhibitors inhibit the various pathwaysused for repair of DNA DSBs (FIG. 2), as described in the Examples aboveand in previous studies (Li et al. 2010). Whether the inhibitors induceapoptosis or inhibit cell cycle progression by fluorescence-activatedflow cytometry analyses (FACS) and annexin V staining, which is anindicator of an early apoptotic stage, and propidium iodide staining, anindicator of plasma membrane permeability that develops at a laterapoptotic stage may also be investigated. These studies may be used toelucidate the mechanism by which the inhibitors inhibit growth of cancercells and enhance cancer cell sensitivity to CRT.

Investigation of the Effects of SUMOylation Inhibitors in GenotoxicStress in Xenograph Models.

Compound 1 and the effective derivatives generated according to themethods described in the Examples above will be tested in cellularassays using colorectal cancer-bearing xenograft models or primarycancer tissues from clinical cancer tissue samples. Mice areadministered drugs by oral gavage, intraperitoneal injection,intravenous injection, or osmotic pump methods. Tumor growth ismonitored by animal body mass scoring and direct measurement of the sizeof tumors. To examine the effect of the inhibitors on enhancingsensitivity of xenograft tumors to radiation, multiple-fractionradiation may be delivered at ˜2.0 Gy per day for 5 consecutive days,and the radiation beam will be focused only on the tumor. To examine theeffect of the inhibitors on sensitization to chemotherapeutic drugs, thedrugs (i.e. doxorubicin, 5-fluorouracil, camptosar or eloxatin) thatshow a synergistic effect with SUMO E1 inhibitor in cellular studiesshould be given together with the SUMO E1 inhibitor. Mice injected withvehicle control (or compound 2) or one of the candidate SUMOylationinhibitors will be compared. Along with these studies, the concentrationof the inhibitors in tumors can be determined over the same period oftime to obtain pharmacokinetic data. SUMOylation levels in the tumorlysates will be analyzed at the same time to establish the direct linkbetween inhibitor treatment and SUMOylation.

SUMO E1 inhibitors should sensitize CRT in these preclinical studiesusing animal models. Such results will establish a new paradigm intargeting SUMO E1 as a new cancer therapeutic strategy. In addition,screening the panel of 60 cancer cell lines using the inhibitor mayelucidate new genetic interactions of SUMO E1 with genes whose mutationscause cancer. It is not expected that these inhibitors would havesignificant toxicity to normal tissues, because lead compound 1 did notcause significant toxicity to human hepatocytes, even when used atconcentrations up to 50 μM (FIG. 9).

Despite the K_(d) of 1 being approximately 180 nM, global SUMOylationwas inhibited with μM concentrations of the inhibitor. This discrepancyis likely due to 1) the long life spans of some SUMOylated proteins and2) the high enzymatic activity of E1 and reversible inhibition by thecompound, so that a small fraction of active E1 can catalyze significantSUMOylation. This scenario also explains the low toxicity of theinhibitor to normal cells that is not as dependent on SUMOylation assome cancer cells (Luo et al. 2009).

Example 8 Derivative 1 Also Possess Anti-HIV Activities

VSV-G pseudotyped pNL43LUCR⁻E⁻, an HIV plasmid which has a luciferasegene inserted in the NEF position and with frameshift mutations in theENV and VPR genes was used to test infectivity of HIV after treatmentwith SUMO inhibitors. This virus is competent for a single round ofreplication, and allows for quantitative measurement of infectivity.HeLa cells were treated with the SUMO E1 inhibitor MLS0207587 for 2 hrsbefore pNL43LUCR⁻E⁻ was introduced. Luciferase activity was collectedafter 48 hrs and compared to untreated cells. The lead SUMO inhibitorMLS-0207587 inhibited HIV infection in a dose dependent manner (FIG.14). This mechanism of this inhibition was due to the SUMO inhibitorpreventing integration of the HIV viral genome to the host genome,thereby preventing replication of HIV.

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What is claimed is:
 1. A SUMOylation inhibitor compound comprising astructure comprising

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein: A and B are independently selected from C, N orheteroatoms; R₁ is H, alkyl, haloalkyl, alkyl-OR₁, aryl, heterocyclyl,heteroaryl, benzyl, alkyl-aryl wherein R₁ is optionally substituted withone to four R₄ groups; R₂ is H or L-R₅, wherein L is —C(O)— or —S(O2)-,and R₅ is alkyl, haloalkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl,benzyl, alkyl-aryl where R₅ is optionally substituted with one to fourR₄ groups; R₃ is CN or —C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl,haloalkyl, aryl, heteroaryl where R₅ is optionally substituted with oneto four R₄ groups; and R₄ is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂,—S(O₂)N(R⁷)₂, —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,—C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,—N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl, alkyl-OR⁷,or alkyl-N(R)₂, where each R⁷ is independently of H, alkyl, haloalkyl,cycloalkyl, heterocyclyl, aryl or heteroaryl; or

and pharmaceutically acceptable derivatives, solvates, salts andstereoisomers thereof; wherein R₁ and R₂ are independently selected fromthe group consisting of H, —S(O)₂—R₈, —CN and —C(O)—X—R₉; wherein R₈ isan alkyl or aryl; wherein R₉ is an alkyl, aryl, or heteroaryl; wherein Xis selected from the group consisting of C, O, N, S or P; wherein R₃ isselected from the group consisting of an alkyl, an alkenyl, and analkylaryl; wherein R₄, R₅, R₆ and R₇ are selected from the groupconsisting of H, a halogen, and an alkyl; wherein X₁ is selected fromthe group consisting of C, N, O, S, and P; and wherein X₂, X₃ and X₄ areselected from the group consisting of N and O; or

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein: A and B are independently selected from the groupconsisting of C, N or heteroatoms; R₁ is H, alkyl, haloalkyl, alkyl-OR₁,aryl, heterocyclyl, heteroaryl, benzyl, alkyl-aryl where R₁ isoptionally substituted with one to four R₄ groups; R₂ is H or L-R₅,wherein L is —C(O)— or —S(O2)-, and R₅ is alkyl, haloalkyl, cycloalkyl,aryl, heterocyclyl, heteroaryl, benzyl, alkyl-aryl where R₅ isoptionally substituted with one to four R₄ groups; R₃: is CN or—O(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl, haloalkyl, aryl,heteroaryl where R₅ is optionally substituted with one to four R₄groups; and R₄: is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂,—S(O₂)N(R⁷)₂, —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,—C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,—N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl, alkyl-OR⁷,or alkyl-N(R)₂, where each R⁷ is independently of H, alkyl, haloalkyl,cycloalkyl, heterocyclyl, aryl or heteroaryl.
 2. The SUMOyationinhibitor compound of claim 1, wherein the structure of the compoundcomprises

wherein R₁ is selected from CN, SO₂Tol or CONHBu; wherein R₂ is selectedfrom COAr or SO₂Ph; and wherein R₃ is selected from Bu, Bn or allyl. 3.The compound of claim 2, wherein the compound is selected from the grouphaving the structure of:


4. The compound of claim 2, wherein the compound is


5. The compound of claim 2, wherein the compound is


6. A method for inhibiting a SUMOylation enzyme in a cell, comprisingadministering, to the cell, a compound comprising a structurecomprising:

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein: A and B are independently selected from C, N orheteroatoms; R₁ is H, alkyl, haloalkyl, alkyl-OR₁, aryl, heterocyclyl,heteroaryl, benzyl, alkyl-aryl wherein R₁ is optionally substituted withone to four R₄ groups; R₂ is H or L-R₅, wherein L is —C(O)— or —S(O2)-,and R₅ is alkyl, haloalkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl,benzyl, alkyl-aryl where R₅ is optionally substituted with one to fourR₄ groups; R₃ is CN or —C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl,haloalkyl, aryl, heteroaryl where R₅ is optionally substituted with oneto four R₄ groups; and R₄ is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂,—S(O₂)N(R⁷)₂, —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,—C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,—N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl, alkyl-OR⁷,or alkyl-N(R)₂, where each R⁷ is independently of H, alkyl, haloalkyl,cycloalkyl, heterocyclyl, aryl or heteroaryl; or

and pharmaceutically acceptable derivatives, solvates, salts andstereoisomers thereof; wherein R₁ and R₂ are independently selected fromthe group consisting of H, —S(O)₂—R₈, —CN and —C(O)—X—R₉; wherein R₈ isan alkyl or aryl; wherein R₉ is an alkyl, aryl, or heteroaryl; wherein Xis selected from the group consisting of C, O, N, S or P; wherein R₃ isselected from the group consisting of an alkyl, an alkenyl, and analkylaryl; wherein R₄, R₅, R₆ and R₇ are selected from the groupconsisting of H, a halogen, and an alkyl; wherein X₁ is selected fromthe group consisting of C, N, O, S, and P; and wherein X₂, X₃ and X₄ areselected from the group consisting of N and O; or

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein: A and B are independently selected from the groupconsisting of C, N or heteroatoms; R₁ is H, alkyl, haloalkyl, alkyl-OR₁,aryl, heterocyclyl, heteroaryl, benzyl, alkyl-aryl where R₁ isoptionally substituted with one to four R₄ groups; R₂ is H or L-R₅,wherein L is —C(O)— or —S(O2)-, and R₅ is alkyl, haloalkyl, cycloalkyl,aryl, heterocyclyl, heteroaryl, benzyl, alkyl-aryl where R₅ isoptionally substituted with one to four R₄ groups; R₃: is CN or—C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl, haloalkyl, aryl,heteroaryl where R₅ is optionally substituted with one to four R₄groups; and R₄: is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂,—S(O₂)N(R⁷)₂, —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,—C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,—N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl, alkyl-OR⁷,or alkyl-N(R)₂, where each R⁷ is independently of H, alkyl, haloalkyl,cycloalkyl, heterocyclyl, aryl or heteroaryl.
 7. The method of claim 6,wherein the structure of the compound comprises

wherein R₁ is selected from CN, SO₂Tol or CONHBu; wherein R₂ is selectedfrom COAr or SO₂Ph; and wherein R₃ is selected from Bu, Bn or allyl. 8.The method of claim 7, wherein the compound is selected from the grouphaving the structure of:


9. The method of claim 7, wherein the compound is


10. The method of claim 7, wherein the compound is


11. The method of claim 6, wherein the SUMOylation enzyme is SUMO E1 orSUMO E2.
 12. The method of claim 6, wherein the cell is part of an invivo population of cells in a subject.
 13. The method of claim 12,wherein the population of cells is a tumor, a population of virallyinfected cells, a population of cells associated with heart disease, apopulation of cells associated with a degenerative disease, or apopulation of cells associated with a genetic disease.
 14. The method ofclaim 6, wherein the cell is part of a population of cells grown inculture.
 15. The method of claim 14, wherein the cell is part of aprimary cell line, a secondary cell line or an immortal cell line. 16.The method of claim 15, wherein the cell line is derived from a tumor, adegenerative disease, a genetic disease or a cardiovascular disease. 17.The method of claim 14, wherein the population of cells is virallyinfected.
 18. A method for treating a disease comprising administeringan effective amount of a pharmaceutical composition to a subject havingthe disease, the pharmaceutical composition comprising a compound astructure comprising

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein: A and B are independently selected from C, N orheteroatoms; R₁ is H, alkyl, haloalkyl, alkyl-OR₁, aryl, heterocyclyl,heteroaryl, benzyl, alkyl-aryl wherein R₁ is optionally substituted withone to four R₄ groups; R₂ is H or L-R₅, wherein L is —C(O)— or —S(O2)-,and R₅ is alkyl, haloalkyl, cycloalkyl, aryl, heterocyclyl, heteroaryl,benzyl, alkyl-aryl where R₅ is optionally substituted with one to fourR₄ groups; R₃ is CN or —C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl,haloalkyl, aryl, heteroaryl where R₅ is optionally substituted with oneto four R₄ groups; and R₄ is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂,—S(O₂)N(R⁷)₂, —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,—C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,—N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl, alkyl-OR⁷,or alkyl-N(R)₂, where each R⁷ is independently of H, alkyl, haloalkyl,cycloalkyl, heterocyclyl, aryl or heteroaryl; or

and pharmaceutically acceptable derivatives, solvates, salts andstereoisomers thereof; wherein R₁ and R₂ are independently selected fromthe group consisting of H, —S(O)₂—R₈, —CN and —C(O)—X—R₉; wherein R₈ isan alkyl or aryl; wherein R₉ is an alkyl, aryl, or heteroaryl; wherein Xis selected from the group consisting of C, O, N, S or P; wherein R₃ isselected from the group consisting of an alkyl, an alkenyl, and analkylaryl; wherein R₄, R₅, R₆ and R₇ are selected from the groupconsisting of H, a halogen, and an alkyl; wherein X₁ is selected fromthe group consisting of C, N, O, S, and P; and wherein X₂, X₃ and X₄ areselected from the group consisting of N and O; or

and the pharmaceutically or functionally acceptable derivative,solvates, salts and stereoisomers thereof, including mixtures thereof inall ratios, wherein: A and B are independently selected from the groupconsisting of C, N or heteroatoms; R₁ is H, alkyl, haloalkyl, alkyl-OR₁,aryl, heterocyclyl, heteroaryl, benzyl, alkyl-aryl where R₁ isoptionally substituted with one to four R₄ groups; R₂ is H or L-R₅,wherein L is —C(O)— or —S(O2)-, and R₅ is alkyl, haloalkyl, cycloalkyl,aryl, heterocyclyl, heteroaryl, benzyl, alkyl-aryl where R₅ isoptionally substituted with one to four R₄ groups; R₃: is CN or—C(O)—OR₆, —C(O)—NHR₆, —SO₂R₆ and R₆ is H, alkyl, haloalkyl, aryl,heteroaryl where R₅ is optionally substituted with one to four R₄groups; and R₄: is halo, —OR⁷, —N(R⁷)₂, —S(R⁷)₂, —SO₂(R⁷)₂,—S(O₂)N(R⁷)₂, —S(O)₂OR⁷, —N(R⁷)S(O)₂R⁷, —OS(O)₂R⁷, —C(O)R⁷, —C(O)OR⁷,—C(O)N(R⁷)₂, —OC(O)R⁷, —OC(O)OR⁷, —OC(O)N(R⁷)₂, —N(R⁷)C(O)R⁷,—N(R⁷)C(O)OR⁷, —N(R⁷)C(O)N(R⁷)₂, —CN, —NO₂, alkyl, haloalkyl, alkyl-OR⁷,or alkyl-N(R)₂, where each R⁷ is independently of H, alkyl, haloalkyl,cycloalkyl, heterocyclyl, aryl or heteroaryl.
 19. The method of claim18, wherein the disease is a cancer, a viral infection, a degenerativedisease or a genetic disease.
 20. The method of claim 19, wherein thestructure of the compound comprises

wherein R₁ is selected from CN, SO₂Tol or CONHBu; wherein R₂ is selectedfrom COAr or SO₂Ph; and wherein R₃ is selected from Bu, Bn or allyl. 21.The method of claim 20, wherein the cancer is colorectal cancer,pancreatic cancer, bone cancer or breast cancer.
 22. The method of claim20, wherein the compound is selected from the group having the structureof: